Anti-DAZL Clone 3/11A

Catalogue No. Target Research Areas Applications Size Price (£)
DAZL3/11A-100UG Deleted in azoospermia-like Endocrinology
Fertility
Genetics
Developmental biology
Stem cell
WB
IHC
IF
0.1mg 300.00

Description

Deleted in Azoospermia-like (DAZL) protein plays a role in germ cell development. Mutations in this gene are linked to spermatogenic failure and infertility in males. Clone 3/11A recognizes DAZL at its C terminus, and is used in detection of DAZL expression by various methods.

Specifications

Form in stock: IgG, purified – 1.0 mg/mL. Also available as unpurified supernatant.
Host: Mouse
Specificity: Recognizes DAZL protein C terminus sequence CRVHHFRRSRAMLKSV.
Human Histology positive control: Ovaries or Testis
Fusion partner: Spleen cells from immunised T/O outbred mice were fused with cells of the SP2/0 myeloma cell line.
Storage: Store at +4°C or -20°C. Avoid repeated freezing and thawing.
Shelf life: 18 months from date of dispatch.
Regulatory/ Restrictions: For research and commercial purposes.

Applications
Application Suggested Dilution
WB 1:100-1:1000
IHC 1:50-1:500
IF 1:50-1:500
References
  1. Bayne, R. A. L., Kinnell, H. L., Coutts, S. M., He, J., Childs, A. J., & Anderson, R. A. (2015). GDF9 is Transiently Expressed in Oocytes before Follicle Formation in the Human Fetal Ovary and is Regulated by a Novel NOBOX Transcript. PLoS ONE, 10(3), e0119819.
  2. Rose, C. M., van den Driesche, S., Sharpe, R. M., Meehan, R. R., & Drake, A. J. (2014). Dynamic changes in DNA modification states during late gestation male germ line development in the rat. Epigenetics & Chromatin, 7, 19.
  3. Chen, S.-R., Zheng, Q.-S., Zhang, Y., Gao, F., & Liu, Y.-X. (2013). Disruption of genital ridge development causes aberrant primordial germ cell proliferation but does not affect their directional migration. BMC Biology, 11, 22.
  4. Anderson, R. A., Fulton, N., Cowan, G., Coutts, S., & Saunders, P. T. (2007). Conserved and divergent patterns of expression of DAZL, VASA and OCT4 in the germ cells of the human fetal ovary and testis. BMC Developmental Biology, 7, 136. IF, Dilution used 1:500
  5. Yamauchi, K., Hasegawa, K., Chuma, S., Nakatsuji, N., & Suemori, H. (2009). In Vitro Germ Cell Differentiation from Cynomolgus Monkey Embryonic Stem Cells. PLoS ONE, 4(4), e5338. IF, Dilution used 1:200

Clone 3/11A used to detect DAZL expression in human ovary cells by Immunofluorescence

(B) Triple fluorescent immunohistochemistry for GDF9 (green), DAZL (blue) and BOLL (red) in 20 week human fetal ovary with DAPI as counterstain (grey). Split channel and merged images in (A) and (B) are shown as are merged images of non-immune serum negative control (NEG). Scale bars are 20μm. (C) Nuclear diameters of DAZL, BOLL and GDF9 stained germ cells indicates that GDF9 positive cells are significantly larger (p<0.001) than DAZL but not BOLL expressing cells (bars indicate mean ± sem). (D) Higher magnification merged image of GDF9/DAZL/BOLL immunohistochemistry showing one large primordial follicle is positive for both GDF9 and DAZL but other follicles are positive only for DAZL. (Bayne, R et al.)
Dilution used: 1:400

Clone 3/11A used to detect DAZL expression in ovarion tissue by Immunofluorescence

Abnormal germ cell proliferation was noted in GR-deficient embryos after sex determination. (Chen, SR et al.)
Dilution used: 1:400

Clone 3/11A used to detect DAZL in ovarian and testicular tissue by Western Blot

Western analysis of 1st and 2nd trimester ovaries and testes. In both ovarian (A) and testicular (B) samples VASA (76 Kd) was not detectable in the 1st trimester samples but was present in those from the 2nd trimester. DAZL (33 Kd) was low/undetectable in 1st trimester ovaries (C) whereas it was detectable in ovarian samples from 2nd trimester and testicular samples from both 1st and 2nd trimester (D). OCT4 (42 Kd) was present in both ovaries (E) and testes (F) during both the 1st and 2nd trimester. The loading control in all cases was β-tubulin (51 Kd). (Anderson, R et al.)
Dilution used: 1:500

Clone 3/11A used to detect DAZL expression in rat testis by IHC-P

Image caption: Immunohistochemistry showing localisation of germ cells and 5mC during mid to late gestation. In order to clearly show the localisation of germ cells at all stages of development… using DAZL as a cytoplasmic marker…. (Rose, C et al.)
Dilution used: 1:500

Clone 3/11A Datasheet

3/11A Publications

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