Anti-Estrogen Receptor ß1 Clone PPG5/10

Catalogue No. Target Research Areas Applications Size Price (£)
PPG5/10-100UG Estrogen Receptor ß1 Endocrinology
Cancer
Fertility
Immunology
Neurosciences
WB
IHC
IF
0.1mg 300.00

Description

Estrogen receptors are commonly over-expressed in cancers. Clone PPG5/10 recognizes Estrogen Receptor Beta (ERβ) 1 and is useful in determining the expression of ERβ1 in cancer cells, mainly via immunohistochemistry.

Specifications

Form in stock: IgG, purified – 1.0 mg/mL. Also available as unpurified supernatant.
Host: Mouse
Specificity: Synthetic peptide CEDSKSKEGSQNPQSQ derived from the C terminus of human ERβ1.
Human Histology positive control: Ovary
Fusion partner: Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Storage: Store at +4°C or -20°C. Avoid repeated freezing and thawing.
Shelf life: 18 months from date of dispatch.
Regulatory/ Restrictions: For research and commercial purposes.

Application
Application Suggested Dilution
Western Blot 1:500 5
Immunohistochemistry 1:5-1:50
Immunofluorescence 1:250
References
  1. Collins, F., MacPherson, S., Brown, P., Bombail, V., Williams, A. R., Anderson, R. A., … Saunders, P. T. (2009). Expression of oestrogen receptors, ERα, ERβ, and ERβ variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERα. BMC Cancer, 9, 330.
  2. Asgari, M., & Morakabati, A. (2011). Estrogen receptor beta expression in prostate adenocarcinoma. Diagnostic Pathology, 6, 61.
  3. Emina Torlakovic, Wolfgang Lilleby, Goran Torlakovic, Sophie D. Fosså, Rajni Chibbar, Prostate carcinoma expression of estrogen receptor-β as detected by PPG5/10 antibody has positive association with primary Gleason grade and Gleason score, Human Pathology, Volume 33, Issue 6, June 2002, Pages 646-651, ISSN 0046-8177.
  4. Schiessl, B., Mylonas, I., Hantschmann, P., Kuhn, C., Schulze, S., Kunze, S., … Jeschke, U. (2005). Expression of Endothelial NO Synthase, Inducible NO Synthase, and Estrogen Receptors Alpha and Beta in Placental Tissue of Normal, Preeclamptic, and Intrauterine Growth-restricted Pregnancies. Journal of Histochemistry and Cytochemistry, 53(12), 1441–1449.
  5. Wong, N. A., Malcomson, R. D., Jodrell, D. I., Groome, N. P., Harrison, D. J. and Saunders, P. T. (2005), ERβ isoform expression in colorectal carcinoma: an in vivo and in vitro study of clinicopathological and molecular correlates. J. Pathol., 207: 53–60.
  6. Rago, V., Romeo, F., Giordano, F., Ferraro, A., Andò, S., & Carpino, A. (2009). Identification of ERβ1 and ERβ2 in human seminoma, in embryonal carcinoma and in their adjacent intratubular germ cell neoplasia. Reproductive Biology and Endocrinology : RB&E, 7, 56.IHC-P, Dilution used 1:100
  7. Ciucci, A., Meco, D., De Stefano, I., Travaglia, D., Zannoni, G. F., Scambia, G., … Gallo, D. (2014). Gender Effect in Experimental Models of Human Medulloblastoma: Does the Estrogen Receptor β Signaling Play a Role? PLoS ONE, 9(7), e101623.IHC-P, Dilution used 1:50
  8. Zannoni G F, Ciucci A, Marucci G, Travaglia D, Stigliano E, Foschini M P, Scambia G & Gallo D. Sexual dimorphism in medulloblastoma features. (2016) Histopathology68, 541–548.IHC-P, Dilution used 1:50

Clone PPG5/10 used to detect expression of ERβ1 in cancer tissue by IHC-P

Image caption: Immunoexpression of ERs in endometrial cancers. Tissues were classified as well (A-D), moderately (E-H) or poorly (J-M) differentiated; main panels show closely adjacent sections from three cancer blocks to allow direct comparisons. (Collins, F et al.)
Dilution used: 1:40

Clone PPG5/10 used to detect expression of ERβ1 in cancer tissue by IHC-P

Image caption: Prostate carcinoma Gleason score 3+3 showing > 70% positive nuclei stained by IHC for ER-b. (Asgari, M et al.)
Dilution used: 1:2

Clone PPG5/10 used to detect expression of ERβ1 in cancer tissue by immunofluorescence

Image caption: Expression of PR paralleled that of ERα not ERβ… C. Fluorescent co-localisation as carried out using antibodies specific for ERα or ERβ1 (both green) and PR (red). The cancers illustrated were classified as well (code 1614), moderately (code 1930) or poorly (c, codes 0001 and 1176) differentiated; at least 8 samples were analysed in each group. Co-expression was detected as yellow/orange immunofluorescence. In the well and moderately differentiated cancers expression of PR was most intense in epithelial cells and broadly overlapped with that of ERα (e.g. in cells indicated by arrows)… (Collins, F et al.)
Dilution used: 1:40

Clone PPG5/10 used to detect expression of ERβ1 in colorectal tissue by Western Blot

Image caption: (A) ERβ1, 2 and 5 mRNA expression (assessed by RT-PCR) and (B) ERβ1 and 2 protein expression (assessed by western blotting) by 10 colorectal carcinoma cell lines… (Wong, N. A et al.)
Dilution used: 1:500

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