Anti-Estrogen Receptor ß5 Clone PPG5/25

Catalogue No. Target Research Areas Applications Size Price (£)
PPG5/25-100UG Estrogen Receptor ß5 Endocrinology
Cancer
Immunology
Neuroscience
Hepatology
WB
IHC
IF
0.1mg 300.00

Description

Estrogen receptors are commonly over-expressed in cancers. Clone PPG5/25 recognizes Estrogen Receptor Beta (ERβ) 5 and is useful in determining the expression of ERβ5 in cancer cells, mainly via immunohistochemistry.

Specifications

Form in stock: IgG, purified – 1.0 mg/mL. Also available as unpurified supernatant.
Host: Mouse
Specificity: Tuberculin conjugated peptide that recognizes C terminus of ERβ5 at sequence LLSHVRHARYAP.
Human Histology positive control: Normal colon or colon carcinoma
Fusion partner: Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Storage: Store at +4°C or -20°C. Avoid repeated freezing and thawing.
Shelf life: 18 months from date of dispatch.
Regulatory/ Restrictions: For research and commercial purposes.

Applications
Application Suggested Dilution
Western Blot Assay dependent
Immunohistochemistry 1:50-1:1001,2,4
Immunofluorescence 1:5003
References
  1. Collins, F., MacPherson, S., Brown, P., Bombail, V., Williams, A. R., Anderson, R. A., … Saunders, P. T. (2009). Expression of oestrogen receptors, ERα, ERβ, and ERβ variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERα. BMC Cancer, 9, 330.
  2. Ciucci, A., Meco, D., De Stefano, I., Travaglia, D., Zannoni, G. F., Scambia, G., … Gallo, D. (2014). Gender Effect in Experimental Models of Human Medulloblastoma: Does the Estrogen Receptor β Signaling Play a Role? PLoS ONE, 9(7), e101623.
  3. LI, S.-Y., WANG, Y.-X., WANG, L., QIAN, Z.-B., & JI, M.-L. (2015). Cytoplasm estrogen receptor β5 as an improved prognostic factor in thymoma and thymic carcinoma progression. Oncology Letters, 10(4), 2341–2346.
  4. Wong, N. A., Malcomson, R. D., Jodrell, D. I., Groome, N. P., Harrison, D. J. and Saunders, P. T. (2005), ERβ isoform expression in colorectal carcinoma: an in vivo and in vitro study of clinicopathological and molecular correlates. J. Pathol., 207: 53–60.
  5. Zannoni G F, Ciucci A, Marucci G, Travaglia D, Stigliano E, Foschini M P, Scambia G & Gallo D (2016) Histopathology68, 541–548. IHC-P, Dilution used 1:10

Clone PPG5/25 used to detect expression of ERβ5 in cancer tissue by IHC-P

Image caption: Immunoexpression of ERs in endometrial cancers. Tissues were classified as well (A-D), moderately (E-H) or poorly (J-M) differentiated; …immunoexpression of ERβ1, 2, 5 was readily detected (K. L, M). Inserts in panels K, L, and M show negative controls for ERβ1, ERβ2 and ERβ5 antibodies respectively generated using primary antibodies pre-absorbed with specific peptides used for immunisation. Asterisks (*) label the stromal compartment that was well defined in the well differentiated cancers. (Collins, F et al.)
Dilution used: 1:75

Clone PPG5/25 used to detect ERβ5 expression in normal human liver by IHC-P

Image caption: Staining pattern of the ERß isoforms in human liver. In order to confirm the specificity of the ERß antibodies used in the study, we immunostained sections of human healthy liver, showing that ERß1 and ERß2 isoforms are not expressed, while specific nuclear ERß5 immunoreactivity is detected (magnification 20x and 40x). This pattern of ERß isoforms immunoreactivity is consistent with previous descriptions. (Ciucci, A et al.)
Dilution used: 1:100

Clone PPG5/25 used to detect expression of ERβ5 in colorectal tissue by Western Blot

Image caption: (A) ERβ1, 2 and 5 mRNA expression (assessed by RT-PCR) and (B) ERβ1 and 2 protein expression (assessed by western blotting) by 10 colorectal carcinoma cell lines… (Wong, N. A et al.)
Dilution used: 1:500

Clone PPG5/25 used to detect ERβ5 expression thymic tumors by Immunofluorescence

Image caption: …(A) …Overexpression of cytoplasmic ERβ5 was observed in thymic carcinomas and thymomas, compared with the normal thymic tissues. (B) ERβ5 expression was observed in the T1682 and TC1889 cell lines through indirect immunofluorescence. (C) …The results revealed that the mean level of ERβ5 gene expression was lower in advanced clinical stages. ERβ5, estrogen receptor β5. (LI, S et al.)
Dilution used: 1:500

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