Anti-Follistatin Clone H10

Catalogue No. Target Research Areas Applications Size Price (£)
H10-100UG Follistatin
(raised to FS315)
Endocrinology
Cancer
Fertility
Immunology
Neurosciences
Stem cell
ELISA
WB
IHC
0.1mg 300.00

Description

Follistatin is a single-chain glycosylated protein that inhibits follicle stimulating hormone (FSH) release.  Alternative splicing of Follistatin mRNA yields two isoforms, FS315 and FS288. FS315 is considered the main circulating form of Follistatin. Clone H10 recognizes an epitope on the C-terminal region of human Follistatin 315, allowing for detection of FSH levels using various analysis methods. ELISA experiments showed that H10 interacts with FS315 and does not interact with FS288. (McPherson, S.J. et al. 1999)

Specifications

Form in stock: IgG, purified – 1.0 mg/mL. Also available as unpurified supernatant.
Host: Mouse
Specificity: Recognizes human Follistatin isoform FS288, raised against recombinant human FS288.
Human Histology positive control: Testis
Fusion partner: Spleen cells immunised from immunised Balb/c mice were fused with cells of the SP2/0 myeloma cell line.
Storage: Store at +4°C or -20°C. Avoid repeated freezing and thawing.
Shelf life: 18 months from date of dispatch.
Regulatory/ Restrictions: For research and commercial purposes.

Applications
Application Suggested Dilution
Western Blot 1 μg/mL 1
Immunohistochemistry – Paraffin 1:100 2
ELISA Assay dependent
References
  1. Stephen J. McPherson, Sally L. Mellor, Hong Wang, Lee W. Evans, Nigel P. Groome, Gail P. Risbridger; Expression of Activin A and Follistatin Core Proteins by Human Prostate Tumor Cell Lines. Endocrinology 1999; 140 (11): 5303-5309.
  2. Fitzgerald, A. M., Benz, C., Clark, A. F., & Wordinger, R. J. (2012). The Effects of Transforming Growth Factor-β2 on the Expression of Follistatin and Activin A in Normal and Glaucomatous Human Trabecular Meshwork Cells and Tissues. Investigative Ophthalmology & Visual Science53(11), 7358–7369.
  3. Belinda Cancilla, Renea A Jarred, Hong Wang, Sally L Mellor, Gerald R Cunha, Gail P Risbridger, Regulation of Prostate Branching Morphogenesis by Activin A and Follistatin, Developmental Biology, Volume 237, Issue 1, 2001, Pages 145-158, ISSN 0012-1606.IHC-P, Dilution used 5 μg/mL

Clone H10 used to detect immunoreactivity with FS315 by IHC-P.

Immunolocalization of FS315 and FS288 to PC3 and LNCaP cell lines. Positive immunoreactivity for FS315 localized to the cytoplasm of the epithelial tumor cell lines LNCaP (A) and PC3 (B) using H10 antibody, no immunoreactivity was present if the antibody was preabsorbed with FS315 peptide (inset Ainset B). Mouse IgG antibody controls were negative (inset Cinset D). (McPherson, S.J. et al. 1999)

Concentration used: 6.75 μg/mL

Western Blots showing binding of H10 to isoforms of FS315 in prostate tumor cell lines

B. FS315 proteins corresponding to 35, 39, and 42K molecular weight isoforms of FS could only be detected by the H10 Ab (Fig. 3B) in lanes containing hrFS315 (lane 3). (McPherson, S.J. et al. 1999)

Concentration used: 1 μg/mL

Clone H10 used to detect expression of FS315 in TM cells by IHC-P

FST 315 expression in NTM (C) and GTM (G) tissues… (Fitzgerald, A et al.)

Dilution used: 1:100

Clone H10 Datasheet

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