Anti-Growth Differentiation Factor 9B Clone 28A

Catalogue No. Target Research Areas Applications Size Price (£)
28A-100UG GDF9B Endocrinology
Cancer
Fertility
Developmental biology
ELISA
WB
ICC
IB
0.1mg 300.00

Description

BMP15, also known as GDF9B, plays a crucial role in the regulation of fertility. Clone 28A has been used to detect BMP15 expression and biosynthesis in various carcinoma progression studies.

Specifications

Form in stock: IgG, purified – 1.0 mg/mL. Also available as unpurified supernatant.
Host: Mouse
Specificity: Recognizes sequence SAEVTASSSKHSGPENNQC on the C-terminus of human GDF9B (BMP15).
Fusion partner: Spleen cells from immunised T/O mice were fused with cells of the SP2/0 myeloma cell line.
Storage: Store at +4°C or -20°C. Avoid repeated freezing and thawing.
Shelf life: 18 months from date of dispatch.
Regulatory/ Restrictions: For research and commercial purposes.

Applications
Application Suggested Dilution
ELISA Assay dependent
Western Blot 1:50001
Immunocytochemistry Assay dependent
Immunoblotting 1:100003
References
  1. Sara L. Al-Musawi, Kelly L. Walton, Derek Heath, Courtney M. Simpson, Craig A. Harrison; Species Differences in the Expression and Activity of Bone Morphogenetic Protein 15. Endocrinology2013; 154 (2): 888-899.
  2. Sudiman, J., Sutton-McDowall, M. L., Ritter, L. J., White, M. A., Mottershead, D. G., Thompson, J. G., & Gilchrist, R. B. (2014). Bone Morphogenetic Protein 15 in the Pro-Mature Complex Form Enhances Bovine Oocyte Developmental Competence. PLoS ONE, 9(7), e103563.
  3. Pulkki, M.M., Myllymaa, S., Pasternack, A., Lun, S., Al-Qahtani, Korchynskyi, O., Groome, N., Juengel, J.L., Laitinen, M., Ritvos, O., Mottershead, D.G. 2011 The bioactivity of human BMP15 is sensitive to C-terminal modification : characterization of the purified untagged processed mature region. Mol. Cell. Endocrinol. 332: 106-15.

Clone 28A used to detect prodomain mutations in human MBP15 biosynthesis by Western Blot

Samples were detected under reduced conditions using a human-specific BMP15 mAb (A) or an anti-FLAG M2 antibody (B). C, Five nonconserved residues (boldface type, underlined) within the α1-helix of the hBMP15 prodomain (Leu44, Glu46, Glu47, Leu49, and Glu50) were substituted with the corresponding mBMP15 residues (Ser43, Leu45, Asp46, Ala48, and Lys49) using site-directed mutagenesis. (Al-Musawi, S et al.)
Dilution used: 1:5000

Clone 28A used to purifiy and quantify human BMP15 by Western Blot

B. The processed mature region of pro-mature BMP15 was quantified by Western blotting [mab28 monoclonal antibody [42]] using the mature region of hBMP15 (R&D Systems) as a standard. Lanes 1–4: mature region of BMP15 (R&D Systems); 200, 100, 50 and 10 ng, respectively. Lanes 5–7: decreasing doses of the purified BMP15 pro-mature complex. (Sudiman, J et al.)

Clone 28A used to detect BMP15 in HEK-293T cell lines by Immunoblotting

Image caption: (B) Recombinant proteins produced from stable HEK-293T cell lines and subjected to SDS–PAGE immunoblotting (ECL). 1: BMP15wt, 2: BMP15 form 1, 3: BMP15 form 2 (with C-terminal Flag tag), 4: GDF9 wt (recognised by the GDF9 specific mAb-53 (Gilchrist et al., 2004b)), 5: TGF-β 200 ng. All samples were reduced with 10 mM DTT before running into the gels. The specific BMP15 mAb-28 reveals the 16–17 kDa mature human BMP15 protein. The mAb-28 does not cross-react with GDF9, showing the specificity of the antibody to BMP15. (Pulkki M,M et al.)
Dilution used: 1:10000

Clone 28A Datasheet

28A Publications

Get in touch