Anti-Myelin Basic Protein Clone 98/P12

Catalogue No. Target Research Areas Applications Size Price (£)
98/P12-100UG Myelin Basic Protein (Thr98) Immunology
Neuroscience
Cell biology
ELISA
WB
ICC
0.1mg 300.00

Description

The phosphorylation of myelin basic protein (MBP) has been shown to decrease the ability of MBP to aggregate lipid vesicles and consequently destabilising the compact structure of myelin, a destruction that has been observed in demyelinating diseases such as Multiple Sclerosis. (Yon, M et al. 1995) Clone 98/P12 is a useful detector of phosphorylated MBP by binding to Thr98 of human MBP in the phosphorylated state.

Specifications

Form in stock: IgG, purified – 1.0 mg/mL. Also available as unpurified supernatant.
Host: Mouse
Specificity: Synthetic peptide corresponding to human MBP when phosphorylated at threonine 98.
Human Histology positive control: Brain tissue
Fusion partner: Spleen cells from immunised Balb/c mice were fused with the mouse SP2/0 myeloma cell line.
Storage: Store at +4°C or -20°C. Avoid repeated freezing and thawing.
Shelf life: 18 months from date of dispatch.
Regulatory/ Restrictions: For research and commercial purposes.

Applications
Application Suggested Dilution
ELISA Assay dependent
WB 1:1000-1:20001,2,4,5
ICC 1:4002
References
  1. Michele Yon, Cameron A. Ackerley, Fabrizio G. Mastronardi, Nigel Groome, Mario A. Moscarello, Identification of a mitogen-activated protein kinase site in human myelin basic protein in situ, Journal of Neuroimmunology, Volume 65, Issue 1, March 1996, Pages 55-59, ISSN 0165-5728.
  2. DeBruin, L.S., Haines, J.D., Wellhauser, L.A., Radeva, G., Schonmann, V., Bienzle, D. and Harauz, G. (2005), Developmental partitioning of myelin basic protein into membrane microdomains. J. Neurosci. Res., 80: 211–225. WB, Dilution 1:2000
  3. Michele Yon, Peter White, Nigel Goome, Preparation of a novel monoclonal antibody specific for myelin basic protein phosphorylated on Thr98, Journal of Neuroimmunology, Volume 58, Issue 2, May 1995, Pages 121-129, ISSN 0165-5728. ELISA
  4. Debruin, Lillian S., Jeffery D. Haines, Dorothee Bienzle, and George Harauz. “Partitioning of Myelin Basic Protein into Membrane Microdomains in a Spontaneously Demyelinating Mouse Model for Multiple SclerosisThis Paper Is One of a Selection of Papers Published in This Special Issue, Entitled CSBMCB Membrane Proteins in Health and Disease.” Biochemistry and Cell Biology 84.6 (2006): 993-1005. WB, Dilution used 1:1000
  5. Atkins, C. M., Yon, M., Groome, N. P. and Sweatt, J. D. (1999), Regulation of Myelin Basic Protein Phosphorylation by Mitogen-Activated Protein Kinase During Increased Action Potential Firing in the Hippocampus. Journal of Neurochemistry, 73: 1090–1097. WB, Dilution used 1:2000

Clone 98/P12 used to detect MBP in brain tissue of normal and MS patients by Western Blot

Western Blot reacted with mAb P12. Lane 8, normal; lane 9, MS 75. (Yon, M et al. 1996)
Dilution used: 1:2000

Clone 98/P12 used to stain OL cells and detect MBP levels by ICC

Colocalization of various lipids and proteins in the developing oligodendrocyte… b: Mature oligodendrocytes fixed at 15 DIV. (DeBruin, L.S et al. 2005)
Dilution used: 1:400

ELISA using clone 98/P12 to test its immunoreactivity against synthetic peptides and proteins

(Yon, M et al. 1995)

Clone 98/P12 Datasheet

98/P12 Publications

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