Anti-Myelin Basic Protein Clone MBP1

Catalogue No. Target Research Areas Applications Size Price (£)
MBP1-100UG Myelin Basic Protein
(region 129-138)
Immunology
Neurosciences
Genetics
Infectious diseases
ELISA
WB
IHC
ICC
0.1ml 300.00

Description

Myelin Basic Protein (MBP) is involved in the process of myelination of nerves. MBP Clone 1 recognizes an epitope in the 129-138 region of MBP, useful in clinical diagnosis to detect MBP levels.

Specifications

Form in stock: IgG, purified – 1.0 mg/mL. Also available as unpurified supernatant.
Host: Mouse
Specificity: Reacts with MBP from human, bovine and rat, recognizing epitope 129-138.
Fusion partner: Spleen cells from immunised NIH mice were fused with cells of the NS0 mouse myeloma cell line.
Human Histology positive control: Human brain tissue
Storage: Store at +4°C or -20°C. Avoid repeated freezing and thawing.
Shelf life: 18 months from date of dispatch.
Regulatory/ Restrictions: For research and commercial purposes.

Applications
Application Suggested Dilution
ELISA 1:200-1:1000
WB 1:10-1:200
IHC 1:10
ICC 1:30002
References
  1. Romain Marignier, Adeline Nicolle, Chantal Watrin, Monique Touret, Sylvie Cavagna, Michel Varrin-Doyer, Gaëlle Cavillon, Véronique Rogemond, Christian Confavreux, Jérôme Honnorat, Pascale Giraudon; Oligodendrocytes are damaged by neuromyelitis optica immunoglobulin G via astrocyte injury. Brain 2010; 133 (9): 2578-2591.
  2. Brunner, C., Lassmann, H., Waehneldt, T. V., Matthieu, J.-M. and Linington, C. (1989), Differential Ultrastructural Localization of Myelin Basic Protein, Myelin/Oligodendroglial Glycoprotein, and 2′,3′-Cyclic Nucleotide 3′-Phosphodiesterase in the CNS of Adult Rats. Journal of Neurochemistry, 52: 296–304.
  3. Jatana, Manu, Inderjit Singh, Avtar K. Singh, and Dorothea Jenkins. “Combination of Systemic Hypothermia and N-acetylcysteine Attenuates Hypoxic-Ischemic Brain Injury in Neonatal Rats.” Nature News. Nature Publishing Group, 01 May 2006.
  4. Nigel Groome., Janice Harland, Adrian Dawkes, Preparation and properties of monoclonal antibodies to myelin basic protein and its peptides, Neurochemistry International, Volume 7, Issue 2, 1985, Pages 309-317, ISSN 0197-0186. ELISA, Dilution used 1:1000
  5. Groome NP, Dawkes A, Gales M, Hruby S, Alvord EC Jr. Region-specific immunoassays for human myelin basic protein J Neuroimmunol. 1986 Oct;12(4):253-64. ELISA

MBP Clone 1 used to detect myelin density in the optic nerve by IHC-P

(B) IgGNMO+reduced myelin expression (MBP immunoreactivity). ( Marignier, R et al.)

MBP Clone 1 used to detect MBP expression in myelin sheaths of adult rats by ICC

(Brunner, C et al.)
Dilution used: 1:3000

MBP Clone 1 used to detect myelin expression in neonatal rats (brain tissue) by IHC-P

B: HYP + NAC and Sham groups are not appreciably different in PLP or MBP staining, indicating preservation of myelin expression… (Jatana, M et al.)
Dilution used: 1:200

Clone MBP 1 Datasheet

MBP 1 Publications

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