Custom Monoclonal Antibody Production

Although BioServUK treats each contract as a custom project, most of the following elements are applicable and help to ensure a quality product is produced:

Consultation with client to discuss antigen selection

Discussion of suitable screening methods relevant to end use assay

Project segmentation to evaluate each milestone reached

Full confidentiality assured

Antibody services handbook

Phase

Time Scale (weeks)

Process

I: Antigen design & immunisation 6-10 Design and preparation of protein/cellular antigens
Determination of serum titer in end use assays
II: Cell fusion & screening of candidate colonies 3-6 Hybridoma fusion and consequent ELISA screen to detect positive ‘binders’
Small scale expansion and cryopreservation of initial positives
III: Limiting dilution cloning 3-5 Limiting dilution cloning of parental clones
IV: Expansion of clonal cell line 3-6 Subclones with high specificity expanded
Shipment of hybridoma and up to 5ml of supernatant
Analysis by SDS-PAGE, WB, ELISA and SEC
V: Antibody scale-up 3-6 Milligramme to Gramme quantities
Scale up performed using bioreactor system
Purification using Protein A/G affinity chromatography

Additional Services

  • Phospho-specific polyclonal antibody service
  • Modified, phospho and control peptide synthesis
  • Antibody fragmentation: Fab and Fab’ fragment
  • Customized and extended immunization schedule
  • Western Blot and Immunohistochemistry testing
  • Multi-stage antibody purification

To request a quote, please fill out the form below and we will get back to you in 1-2 business days.  For more information, don’t hesitate to email us at info@bioservuk.com

Antibody Production Enquiry Form

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