Protein Bulk Production Services

Our experience and versatility allows us to work to a scale to meet your requirements.

We provide bulk production of your bacterial, mammalian or insect cell lines

Project segmentation to evaluate each milestone reached

Full confidentiality assured

Custom services handbook

Phase

Standard for Bacterial scale-up

Standard for Mammalian scale-up

I: Receipt of client DNA Clone DNA into a specified vector and transfect it into BL21 DE3 cells Clone DNA into a specified vector and transfect it into HEK293 cells
II: Pilot 50ml production Small scale expression screen

Optimisation of growth/expression parameters followed by pelleting down and collecting cells

Small scale expression screen

Scale-up in shaker flask culture

III: Purification of cells from initial pilot Cell pellets lyzed using a sonicator

Supernatant will be collected and will undergo affinity purification using Nickel or another appropriate affinity tag

Harvesting of cell culture supernatant

Confluent supernatant harvested and concentrated by cross flow filtration

IV: QC assessment of protein purity Analysis of purity by SDS-PAGE, WB, and anti-His WB Analysis of purity by SDS-PAGE, WB, and anti-His WB
V: Scale-up Scale-up based on initial pilot data to meet your requirements

Milligramme to Gramme quantities

Scale-up based on initial pilot data to meet your requirements

Milligramme to Gramme quantities

To request a quote, please fill out the form below and we will get back to you in 1-2 business days.  For more information, don’t hesitate to email us at info@bioservuk.com

Protein Bulk Production Enquiry Form

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