PG-R purified using Protein Ark Ni-NTA HiFliQ column, analysed via SERVA 4-20%, PG-R detected at 7 kDa
Comparison between Protein Ark Ni-NTA resin and a competitor’s Ni-NTA resin to purify Protein G. Protein G was purified from pET15B plasmids expressed in E.coli, strain BL21(DE3). The Ni-NTA resin sourced from Protein Ark yielded PG-R with greater purity than other resins.
Lane 1: SERVA prestained SDS-PAGE Protein Marker 6.5 – 200kDa
Lane 2: Load
Lane 3: Flow-through
Lane 4: Wash (PBS pH 7.5)
Lane 5: Purified PG-R (eluted with 250mM imidazole pH 7.5)
Purification of various IgG subclasses using PG-R, analysed via NuPAGE
Murine hybridoma supernatants containing IgG2a,2b,3 monoclonal antibodies were purified using PG-R.
Lane 1: Molecular weight marker (SeeBluePlus2)
Lane 2: Purified IgG
Sequence: Supplied under NDA.
Molecular Weight: 7 kDa
Form: Liquid, 10 mg/ml in filter sterile PBS.
Storage: Store at +4°C short term (1-2 weeks) or -20°C for longer periods. Avoid repeated freezing and thawing.
Shelf life: 18 months from date of dispatch.
Regulatory/ Restrictions: For research and commercial purposes.