Antibody Bulk Production
Phase |
Process |
---|---|
I : Receipt of client cell line | We will receive your cell line as a frozen ampoule of growing culture
Initial growth in serum supplemented medium followed by mycoplasma analysis using PCR |
II : Pilot 1 L production | Adaptation of cell line onto protein-free medium
Scale-up in roller bottle culture, typical yield of 20-40mg of purified antibody |
III : Downstream processing | Harvesting of cell culture supernatant
Concentration by tangential cross flow filtration Protein A/G purification Final buffer formulation |
IV : QC assessment of antibody purity | Analysis of purity by SDS-PAGE, WB, ELISA and aggregation assessment by SEC |
V : Antibody scale-up | Milligramme to Gramme quantities
Scale up performed using bioreactor system Purification using Protein A/G affinity chromatography |
Final Deliverables
- Purified monoclonal antibody, protein, or concentrated supernatant to requested scale (up to 250L)
- Working Cell Bank for Scale-up cell line
- Project report with ELISA, SDS-PAGE, Western Blot, and SEC performance data
To request a quote, please fill out the form below and we will get back to you in 1-2 business days. For more information, don’t hesitate to email us at info@bioservuk.com
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