Antibody Bulk Production

Using roller bottle technology we provide bulk production of your cell lines quickly and efficiently

Ongoing liaison between client and BioServUK during hybridoma development process



I : Receipt of client cell line
  • We will receive your cell line as a frozen ampoule of growing culture
  • Initial growth in serum supplemented medium followed by mycoplasma analysis using PCR
II : Pilot 1 L production
  • Adaptation of cell line onto protein-free medium
  • Scale-up in roller bottle culture, typical yield of 20-40mg of purified antibody
III : Downstream processing
  • Harvesting of cell culture supernatant
  • Concentration by tangential cross flow filtration
  • Protein A/G purification
  • Final buffer formulation
IV : QC assessment of antibody purity
  • Analysis of purity by SDS-PAGE, WB, ELISA and aggregation assessment by SEC
V : Antibody scale-up
  • Milligramme to Gramme quantities
  • Scale up performed using bioreactor system
  • Purification using Protein A/G affinity chromatography

Final Deliverables

  • Purified monoclonal antibody, protein, or concentrated supernatant to requested scale (up to 250L)
  • Working Cell Bank for Scale-up cell line
  • Project report with ELISA, SDS-PAGE, Western Blot, and SEC performance data

To request a quote, please fill out the form below and we will get back to you in 1-2 business days. For more information, don’t hesitate to email us at

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