Antibody Bulk Production
Using roller bottle technology we provide bulk production of your cell lines quickly and efficiently
Ongoing liaison between client and BioServUK during hybridoma development process
Application note
Custom services handbook
Phase |
Process |
|---|---|
| I : Receipt of client cell line | We will receive your cell line as a frozen ampoule of growing culture
Initial growth in serum supplemented medium followed by mycoplasma analysis using PCR |
| II : Pilot 1 L production | Adaptation of cell line onto protein-free medium
Scale-up in roller bottle culture, typical yield of 20-40mg of purified antibody |
| III : Downstream processing | Harvesting of cell culture supernatant
Concentration by tangential cross flow filtration Protein A/G purification Final buffer formulation |
| IV : QC assessment of antibody purity | Analysis of purity by SDS-PAGE, WB, ELISA and aggregation assessment by SEC |
| V : Antibody scale-up | Milligramme to Gramme quantities
Scale up performed using bioreactor system Purification using Protein A/G affinity chromatography |
Final Deliverables
- Purified monoclonal antibody, protein, or concentrated supernatant to requested scale (up to 250L)
- Working Cell Bank for Scale-up cell line
- Project report with ELISA, SDS-PAGE, Western Blot, and SEC performance data
To request a quote, please fill out the form below and we will get back to you in 1-2 business days. For more information, don’t hesitate to email us at info@bioservuk.com
Comments are closed.