Hydrophobic Interaction Chromatography (HIC) Resins

HIC is a versatile technique and can show high selectivity to individual molecules according to their exposed hydrophobic zones. It is particularly useful for intermediate and final-stage purifications.

Butyl SepFast™, Phenyl SepFast™, and Octyl SepFast™ are hydrophobic interaction chromatography (HIC) adsorbents with carbon chains of C4, C6 (benzene ring), and C8, respectively. They are specially designed for the purification of biological molecules based on their hydrophobicity profiles.

HIC media requires much milder purification conditions than reversed phase chromatography (RPC) media. Hence biological activity can be maintained in HIC separations unlike RPC operations.

HIC media normally binds at moderate to high salt concentrations. It is therefore logical to place a HIC step after an IEX step where molecules are usually eluted at high salt conditions.

SepFast™ HIC media offers a broad range of choices from C4 to C8. In general, the longer the carbon chain, the higher the surface hydrophobicity.

Features of our HIC resins:

  • High sample loading capacity.
  • High separation power.
  • Available at all pack sizes from analytical up to full manufacturing scale.
  • Chemically stable Agarose base matrix.
  • Can be produced in high and low ligand densities, alternate crosslinking levels and different bead sizes to optimise the media to the separation.
  • All versions can be scaled up and produced for GMP use.
  • More choices than other suppliers.

4HF serial (50µm – 150µm)

The above HIC media is designed to purify most medium to large proteins

6HF serial (50µm – 150µm)

The above HIC media is designed to purify peptides or smaller proteins

Large Beads (150µm – 350µm)

The above HIC media is designed to purify proteins from crude or viscous samples

HighRes (25µm – 50µm)

The above HIC media is designed to purify proteins
that require high resolution

Product codeProduct namePack sizeDownloadsPriceBuy
481301-25MLPhenyl SepFast Large Beads (25 mL)25 mL

481301-100MLPhenyl SepFast Large Beads (100 mL)100 mL

481301-1LPhenyl SepFast Large Beads (1 L)1 L

481701-1LOctyl SepFast Large Beads (1 L)1 L

481701-100MLOctyl SepFast Large Beads (100 mL)100 mL

481701-25MLOctyl SepFast Large Beads (25 mL)25 mL

481801-1LOctyl SepFast 6HF HIC media (1 L)1 L

481801-100MLOctyl SepFast 6HF HIC media (100 mL)100 mL

481801-25MLOctyl SepFast 6HF HIC media (25 mL)25 mL

481601-25mLOctyl SepFast 4HF HIC media (25 mL)25 mL

481601-1LOctyl SepFast 4HF HIC media (1 L)1 L

481101-1LPhenyl SepFast HighRes HIC media (1 L)1 L

481101-100MLPhenyl SepFast HighRes HIC media (100 mL)100 mL

481101-25MLPhenyl SepFast HighRes HIC media (25 mL)25 mL

481202-25MLPhenyl SepFast 6HF High Sub HIC media (25 mL)25 mL

481202-100MLPhenyl SepFast 6HF High Sub HIC media (100 mL)100 mL

481202-1LPhenyl SepFast 6HF High Sub HIC media (1 L)1 L

481201-1LPhenyl SepFast 6HF HIC media (1 L)1 L

481201-100MLPhenyl SepFast 6HF HIC media (100 mL)100 mL

481201-25MLPhenyl SepFast 6HF HIC media (25 mL)25 mL

481001-1LPhenyl SepFast 4HF HIC media (1 L)1 L

481001-100MLPhenyl SepFast 4HF HIC media (100 mL)100 mL

481001-25MLPhenyl SepFast 4HF HIC media (25 mL)25 mL

480201-1LButyl SepFast Large Beads (1 L)1 L

480201-100MLButyl SepFast Large Beads (100 mL)100 mL

480201-25MLButyl SepFast Large Beads (25 mL)25 mL

480301-1LButyl SepFast 6HF HIC media (1 L)1 L

480301-100MLButyl SepFast 6HF HIC media (100 mL)100 mL

480301-25MLButyl SepFast 6HF HIC media (25 mL)25 mL

480101-1LButyl SepFast 4HF HIC media (1 L)1 L

480101-100MLButyl SepFast 4HF HIC media (100 mL)100 mL

480101-25MLButyl SepFast 4HF HIC media (25 mL)25 mL

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