Purity: >95 % as determined by SDS-PAGE.
Endotoxin: <1.0 EU per μg protein as determined by the LAL method.
Activity: The 2019-nCoV Spike Protein (RBD, His Tag) can bind with Human ACE2 in functional ELISA assay.
Protein Construction: A DNA sequence encoding the NCP-CoV(2019-nCoV) Spike Protein (RBD) was expressed with a polyhistidine tag at the C-terminus.
Expressed Host: Baculovirus-Insect Cells
Predicted N Terminal: Arg
Molecule Mass: The recombinant NCP-CoV(2019-nCoV) Spike Protein (RBD, His Tag) consists of 234 amino acids and predicts a molecular mass of 26.54 kDa.
Formulation: Lyophilized from sterile 20mM PB,300mM NaCl, pH 7.0,10% glycerol.
Please contact us for any concerns or special requirements.
Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping: Generally, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Regulatory/ Restrictions: For research use only.