||Recombinant SARS-CoV-2 Spike S2 Protein, polyhistidine tag
Recombinant protein of SARS-CoV-2 Spike S2 extracellular domain region from Wuhan pneumonia virus (MN908947.3) , with a polyhistidine tag. This product is still under development due to an extremely low yield.
The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. The SARS-CoV-2 spike (S) protein is composed of two subunits; the S1 subunit contains a receptor-binding domain that engages with the host cell receptor angiotensin-converting enzyme 2 and the S2 subunit mediates fusion between the viral and host cell membranes. The S RBD protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity, during infection with SARS-CoV-2 (2019-nCoV) as in recent COVID-19 outbreak.
Applications: Antigens, Western, ELISA and other in vitro binding or in vivo functional assays, and protein-protein interaction studies.
Purity: >90 % by SDS-PAGE.
Endotoxin: Endotoxin level is < 0.1 ng/μg of protein (<1 EU/μg)
Expressed Host: Expressed and purified from in vitro cell culture of HEK293 cells
Molecule Weight: 59.7 kDa
Formulation: Purified protein formulated in a sterile solution of PBS buffer, pH7.2, without any preservatives.
Storage: The product is shipped with dry ice. Upon receipt, unopened vial can be stored at -80°C for over 12 months. Avoid repeated freeze/thaw cycles. Also the product can be aliquoted in the smaller size of working aliquots with the desired buffer and concentration, and stored at or below -20°C stable for 3 to 4 weeks.
Regulatory/ Restrictions: For research use only.