Catalogue No. Description Expressed Host Size
LTP-V006 Recombinant Human ACE2 Protein (H374N, and H378N), human IgG1 Fc tag HEK293 Cells 50 ug

Recombinant protein of SARS-CoV-2 Spike S1 from Wuhan pneumonia virus (MN908947.3), with a polyhistidine tag.

The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. The SARS-CoV-2 spike (S) protein is composed of two subunits; the S1 subunit contains a receptor-binding domain that engages with the host cell receptor angiotensin-converting enzyme 2 and the S2 subunit mediates fusion between the viral and host cell membranes. The S RBD protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity, during infection with SARS-CoV-2 (2019-nCoV) as in recent COVID-19 outbreak.

Specifications

Applications: Antigens, Western, ELISA and other in vitro binding or in vivo functional assays, and protein-protein interaction studies.
Purity: >90 % by SDS-PAGE.
Endotoxin: Endotoxin level is < 0.1 ng/μg of protein (<1 EU/μg) Expressed Host: Expressed and purified from in vitro cell culture of HEK293 cells
Species: 2019-nCoV
Molecule Weight: 77.1 kDa
Formulation: Purified protein formulated in a sterile solution of PBS buffer, pH7.2, without any preservatives.
Storage: The product is shipped with dry ice. Upon receipt, unopened vial can be stored at -80°C for over 12 months. Avoid repeated freeze/thaw cycles. Also the product can be aliquoted in the smaller size of working aliquots with the desired buffer and concentration, and stored at or below -20°C stable for 3 to 4 weeks.
Regulatory/ Restrictions: For research use only.

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