Custom Monoclonal Antibody Production

Although BioServUK treats each contract as a custom project, most of the following elements are applicable and help to ensure a quality product is produced:

Consultation with client to discuss antigen selection

Discussion of suitable screening methods relevant to end use assay

Project segmentation to evaluate each milestone reached

Full confidentiality assured


Time Scale


I: Antigen design & immunisation 6-10 Weeks
  • Design and preparation of protein/cellular antigens
  • Determination of serum titer in end use assays
II: Cell fusion & screening of candidate colonies 3-6 Weeks
  • Hybridoma fusion and consequent ELISA screen to detect positive ‘binders’
  • Small scale expansion and cryopreservation of initial positives
III: Limiting dilution cloning 3-5 Weeks
  • Limiting dilution cloning of parental clones
IV: Expansion of clonal cell line 3-6 Weeks
  • Subclones with high specificity expanded
  • Shipment of hybridoma and up to 5ml of supernatant
  • Analysis by SDS-PAGE, WB, ELISA and SEC
V: Antibody scale-up 3-6 Weeks
  • Milligramme to Gramme quantities
  • Scale up performed using bioreactor system
  • Purification using Protein A/G affinity chromatography

Additional Services

  • Phospho-specific polyclonal antibody service
  • Modified, phospho and control peptide synthesis
  • Antibody fragmentation: Fab and Fab’ fragment
  • Customized and extended immunization schedule
  • Western Blot and Immunohistochemistry testing
  • Multi-stage antibody purification

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