Custom Monoclonal Antibody Production

Although BioServUK treats each contract as a custom project, most of the following elements are applicable and help to ensure a quality product is produced:

Phase	Time Scale	Process
I: Antigen design & immunisation	6-10 Weeks	Design and preparation of protein/cellular antigens Determination of serum titer in end use assays
II: Cell fusion & screening of candidate colonies	3-6 Weeks	Hybridoma fusion and consequent ELISA screen to detect positive ‘binders’ Small scale expansion and cryopreservation of initial positives
III: Limiting dilution cloning	3-5 Weeks	Limiting dilution cloning of parental clones
IV: Expansion of clonal cell line	3-6 Weeks	Subclones with high specificity expanded Shipment of hybridoma and up to 5ml of supernatant Analysis by SDS-PAGE, WB, ELISA and SEC
V: Antibody scale-up	3-6 Weeks	Milligramme to Gramme quantities Scale up performed using bioreactor system Purification using Protein A/G affinity chromatography

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