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CYP450 Aromatase

CYP450 Aromatase is part of the CYP19A1 family involved in the aromatization of androgens to estrogens, a highly conserved mechanism amongst mammals. Clone H4 recognizes a conserved epitope on the CYP450 aromatase, allowing for detection of aromatase levels using various analysis methods. (Turner et al. 2002).

Catalogue No. Applications Size Price
H4 WB, IHC, IF, ICC 0.1ml

Clone H4 used to detect aromatase in human tissues by IHC-P.

A: Intense aromatase immunostaining in IGCN cells.
B: Placental-like alkaline phosphatase staining of IGCN basal cells
C: Strong aromatase immunoreactivity of interstitial Leydig cells in normal testis (Lc)
D: Intense immunostaining of luteal cells (Luc) in ovarian tissue.(Rago, V et al. 2005)

Dilution used: 1:50

Binding of H4 to a 55 kDa protein on the Cytochrome P450 aromatase protein by Western Blot.

On Western blots, a single protein of _55 kDa was recognised by the monoclonal antibody raised against a peptide within the P450 aromatase protein (A). No signal was seen on blots incubated with antibody that had been pre-absorbed with immunising peptide (B). Extracts were prepared from the following tissues: lane 1, human placenta; lane 2, marmoset placenta; lane 3, marmoset ovary; lane 4, ovary from pregnant rat; lanes 5 and 6, ovaries from two rats killed at pro-oestrus.(Turner et al. 2002).

Dilution used: 1:5000

Clone H4 used to detect aromatase in pig sperm by Immunofluorescence.

B) P450arom red brilliant light in the proximal tail of sperm with a diffuse labelling in the distal tail…. (Rago, V et al. 2007)

Dilution used: 1:100

Clone H4 used to detect aromatase in stromal cells by ICC.

Immunocytochemical localization of aromatase in the stromal cells of an endometriotic lesion (B) and in granulosa cells of a developing follicle (C, positive control). The histology of this lesion (A). st = stromal cells. (Fazleabas,, AT et al.)

Dilution used: 1:75

Product Name Anti Human Cytochrome P450 H4 Aromatase
Clone, Isotype H4, IgG2a
Format 10 x s/n
Tested Applications WB, IHC-P, IF, ICC

Form in Stock: 10 x s/n (concentrated supernatant)
Host: Mouse
Specificity: Synthetic peptide corresponding to AAs 376-390 of human aromatase(KALEDDVIDGYPVKK)
Human Histology positive control: Human placenta
Fusion Partner: Spleen cells immunised from Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage: Store at +4°C or -20°C. Avoid repeated freezing and thawing
Shelf life: 18 months from date of dispatch
Regulatory information: Fit for research and commercial purposes.

  1. Turner, KJ, S. Macpherson, MR Milar, AS McNeilly, K. Williams, M. Cranfield, NP Groome, RM Sharpe, HM Fraser, and PT Saunders. “Development and Validation of a New Monoclonal Antibody to Mammalian Aromatase.” JOE. J Endocrinol, 2002.
  2. Rago V, Romeo F, Aquila S, Montanaro D, Andò S, Carpino A. Cytochrome P450 aromatase expression in human seminoma. Reproductive Biology and Endocrinology. 2005;3:72. doi:10.1186/1477-7827-3-72.
  3. Rago, V., Aquila, S., Panza, R., & Carpino, A. (2007). Cytochrome P450arom, androgen and estrogen receptors in pig sperm. Reproductive Biology and Endocrinology5, 23. http://doi.org/10.1186/1477-7827-5-23.
  4. Asgerally T Fazleabas, Allison Brudney, Daniel Chai, David Langoi, Serdar E Bulun, Steroid receptor and aromatase expression in baboon endometriotic lesions, Fertility and Sterility, Volume 80, Supplement 2, September 2003, Pages 820-827, ISSN 0015-0282, https://doi.org/10.1016/S0015-0282(03)00982-8.
  5. Sirianni, R., Chimento, A., De Luca, A., Zolea, F., Carpino, A., Rago, V., … Pezzi, V. (2009). Inhibition of Cyclooxygenase-2 Down-regulates Aromatase Activity and Decreases Proliferation of Leydig Tumor Cells. The Journal of Biological Chemistry284(42), 28905–28916. http://doi.org/10.1074/jbc.M109.041020. WB, Dilution used 1:50
  6. Catalano, S., Malivindi, R., Giordano, C., Gu, G., Panza, S., Bonofiglio, D., … Andò, S. (2010). Farnesoid X Receptor, through the Binding with Steroidogenic Factor 1-responsive Element, Inhibits Aromatase Expression in Tumor Leydig Cells. The Journal of Biological Chemistry285(8), 5581–5593. http://doi.org/10.1074/jbc.M109.052670. IF, Dilution used 1:100
  7. Bruce K. Campbell, M. Clinton, R. Webb; The Role of Anti-Müllerian Hormone (AMH) During Follicle Development in a Monovulatory Species (Sheep). Endocrinology 2012; 153 (9): 4533-4543. doi: 10.1210/en.2012-1158. IHC-P
  8. S Wilsher, F Stansfield, R E S Greenwood, P D Trethowan, R A Anderson, F B W Wooding, and W R Allen,Ovarian and placental morphology and endocrine functions in the pregnant giraffe (Giraffa camelopardalis) Reproduction 145 (6) 541-554, doi: 10.1530/REP-13-0060 First published online 2 April 2013. WB, Dilution used 1:5000
  9. Tomi Pakarainen, Fu-Ping Zhang, Laura Nurmi, Matti Poutanen, Ilpo Huhtaniemi; Knockout of Luteinizing Hormone Receptor Abolishes the Effects of Follicle-Stimulating Hormone on Preovulatory Maturation and Ovulation of Mouse Graafian Follicles. Mol Endocrinol 2005; 19 (10): 2591-2602. doi: 10.1210/me.2005-0075. IHC-P, Dlution used 1:50
  10. Wieslawa Mlodawska, Maria Slomczynska, Immunohistochemical localization of aromatase during the development and atresia of ovarian follicles in prepubertal horses, Theriogenology, Volume 74, Issue 9, December 2010, Pages 1707-1712, ISSN 0093-691X, https://doi.org/10.1016/j.theriogenology.2010.04.019. IHC-P, Dilution used 1:50

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