Our Standard Protein Expression & Purification Stages:
Phase |
Time Scale |
Process |
---|---|---|
I: Gene synthesis & preparation of target vector | 3 Weeks |
|
IIa: Small scale expression | 2-3 Weeks |
|
IIb: Medium scale expression | 2-3 Weeks |
|
III: Large scale expression | 3-6 Weeks |
|
Additional Services
Bacterial:
Phase I
Standard vector: pET
Standard host cell: BL21 DE3
Phase IIa
- 50ml screens
- Optimal growth and expression parameters are achieved through the application of a range of different temperature, media & bacterial strains
Mammalian:
Phase I
Standard vector: pcDNA3.1
Standard host cell: HEK 293
Phase IIa
- Transient expression tests
- Protein can be expressed intracellularly or secreted into media, depending on protein
Insect:
Phase I
Standard vector: pOET1
Standard host cell: Baculovirus
Phase IIa
- Amplification of virus stock (100-200ml)
- Test expression in Sf9 and Tni cells by Western Blot analysis
Phase III
- Amplification & QPCR titration of virus
- Purification using His tag
To request a quote, please fill out the form below and we will get back to you in 1-2 business days. For more information, don’t hesitate to email us at info@bioservuk.com