Anti-Estrogen Receptor ß1 Clone PPG5/10

Estrogen receptors are commonly over-expressed in cancers. Clone PPG5/10 recognizes Estrogen Receptor Beta (ERβ) 1 and is useful in determining the expression of ERβ1 in cancer cells, mainly via immunohistochemistry.

Catalogue No. Target Research Areas Applications Size Price (£)
PPG5/10-100UG Estrogen Receptor ß1 Endocrinology
Cancer
Fertility
Immunology
Neurosciences
WB
IHC
IF
0.1mg 300.00

Clone PPG5/10 used to detect expression of ERβ1 in cancer tissue by IHC-P

Image caption: Immunoexpression of ERs in endometrial cancers. Tissues were classified as well (A-D), moderately (E-H) or poorly (J-M) differentiated; main panels show closely adjacent sections from three cancer blocks to allow direct comparisons. (Collins, F et al.)
Dilution used: 1:40

Clone PPG5/10 used to detect expression of ERβ1 in cancer tissue by IHC-P

Image caption: Prostate carcinoma Gleason score 3+3 showing > 70% positive nuclei stained by IHC for ER-b. (Asgari, M et al.)
Dilution used: 1:2

Clone PPG5/10 used to detect expression of ERβ1 in cancer tissue by immunofluorescence

Image caption: Expression of PR paralleled that of ERα not ERβ… C. Fluorescent co-localisation as carried out using antibodies specific for ERα or ERβ1 (both green) and PR (red). The cancers illustrated were classified as well (code 1614), moderately (code 1930) or poorly (c, codes 0001 and 1176) differentiated; at least 8 samples were analysed in each group. Co-expression was detected as yellow/orange immunofluorescence. In the well and moderately differentiated cancers expression of PR was most intense in epithelial cells and broadly overlapped with that of ERα (e.g. in cells indicated by arrows)… (Collins, F et al.)
Dilution used: 1:40

Clone PPG5/10 used to detect expression of ERβ1 in colorectal tissue by Western Blot

Image caption: (A) ERβ1, 2 and 5 mRNA expression (assessed by RT-PCR) and (B) ERβ1 and 2 protein expression (assessed by western blotting) by 10 colorectal carcinoma cell lines… (Wong, N. A et al.)
Dilution used: 1:500

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