Anti-Estrogen Receptor ß2 Clone 57/3

Estrogen receptors are commonly over-expressed in cancers. Clone 57/3 recognizes Estrogen Receptor Beta (ERβ) 2 and is useful in determining the expression of ERβ2 in cancer cells via various analysis methods.

Catalogue No. Target Research Areas Applications Size Price (£)
57/3-100UG Estrogen Receptor ß2 Endocrinology
Cancer
Fertility
Immunology
Hepatology
WB
IHC
IF
0.1mg 300.00

Clone 57/3 used to detect expression of ERβ2 in cancer tissue by IHC-P

Image caption: Immunoexpression of ERs in endometrial cancers. Tissues were classified as well (A-D), moderately (E-H) or poorly (J-M) differentiated; …immunoexpression of ERβ1, 2, 5 was readily detected (K. L, M). Inserts in panels K, L, and M show negative controls for ERβ1, ERβ2 and ERβ5 antibodies respectively generated using primary antibodies pre-absorbed with specific peptides used for immunisation. Asterisks (*) label the stromal compartment that was well defined in the well differentiated cancers. (Collins, F et al.)
Dilution used: 1:30

Clone 57/3 used to detect ERβ2 expression in normal human liver by IHC-P

Image caption: Staining pattern of the ERß isoforms in human liver. In order to confirm the specificity of the ERß antibodies used in the study, we immunostained sections of human healthy liver, showing that ERß1 and ERß2 isoforms are not expressed, while specific nuclear ERß5 immunoreactivity is detected (magnification 20x and 40x). This pattern of ERß isoforms immunoreactivity is consistent with previous descriptions. (Ciucci, A et al.)
Dilution used: 1:100

Clone 57/3 used to detect expression of ERβ2 in Leydig cell tumors by IHC-P

Image caption: Morphology and immunolocalization of aromatase and estrogen receptors in Leydig cell tumors (A and B) and control testis (C). A–C: Hematoxylin–eosin staining. A1–C1: Aromatase immunolocalization. A2–C2: ERα immunoreactivity. A3–C3: ERβ1 immunodetection. A4–C4: ERβ2 immunolocalization. L, Leydig cells; T, seminiferous tubule. (Carpino, A et al.)
Dilution used: 1:100

Clone 57/3 used to detect expression of ERβ2 in colorectal tissue by Western Blot

Image caption: (A) ERβ1, 2 and 5 mRNA expression (assessed by RT-PCR) and (B) ERβ1 and 2 protein expression (assessed by western blotting) by 10 colorectal carcinoma cell lines… (Wong, N. A et al.)
Dilution used: 1:500

Clone 57/3 used to detect expression of ERβ2 in ovarian cancer cells by Immunofluorescence

Image caption: A) Representative pictures for ERα, ERβ1, ERβ2, ERβ5, PR, and AR immunostaining in LGSOC patients (magnification 20 ×) and bar chart showing levels of hormone receptor expression (mean ± SEM, n = 25). Results are expressed as IRS (Immunoreactive receptor score). B) Representative pictures showing exclusive nuclear and nuclear/cytoplasmic ERβ2 expression in primary tumor and matched peritoneal metastasis.(Buttarelli, M et al.)
Dilution used: 1:100

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