Anti-Estrogen Receptor ß5 Clone PPG5/25

Estrogen receptors are commonly over-expressed in cancers. Clone PPG5/25 recognizes Estrogen Receptor Beta (ERβ) 5 and is useful in determining the expression of ERβ5 in cancer cells, mainly via immunohistochemistry.

Catalogue No. Target Research Areas Applications Size Price (£)
PPG5/25-100UG Estrogen Receptor ß5 Endocrinology
0.1mg 347.00

Clone PPG5/25 used to detect expression of ERβ5 in cancer tissue by IHC-P

Image caption: Immunoexpression of ERs in endometrial cancers. Tissues were classified as well (A-D), moderately (E-H) or poorly (J-M) differentiated; …immunoexpression of ERβ1, 2, 5 was readily detected (K. L, M). Inserts in panels K, L, and M show negative controls for ERβ1, ERβ2 and ERβ5 antibodies respectively generated using primary antibodies pre-absorbed with specific peptides used for immunisation. Asterisks (*) label the stromal compartment that was well defined in the well differentiated cancers. (Collins, F et al.)
Dilution used: 1:75

Clone PPG5/25 used to detect ERβ5 expression in normal human liver by IHC-P

Image caption: Staining pattern of the ERß isoforms in human liver. In order to confirm the specificity of the ERß antibodies used in the study, we immunostained sections of human healthy liver, showing that ERß1 and ERß2 isoforms are not expressed, while specific nuclear ERß5 immunoreactivity is detected (magnification 20x and 40x). This pattern of ERß isoforms immunoreactivity is consistent with previous descriptions. (Ciucci, A et al.)
Dilution used: 1:100

Clone PPG5/25 used to detect expression of ERβ5 in colorectal tissue by Western Blot

Image caption: (A) ERβ1, 2 and 5 mRNA expression (assessed by RT-PCR) and (B) ERβ1 and 2 protein expression (assessed by western blotting) by 10 colorectal carcinoma cell lines… (Wong, N. A et al.)
Dilution used: 1:500

Clone PPG5/25 used to detect ERβ5 expression thymic tumors by Immunofluorescence

Image caption: …(A) …Overexpression of cytoplasmic ERβ5 was observed in thymic carcinomas and thymomas, compared with the normal thymic tissues. (B) ERβ5 expression was observed in the T1682 and TC1889 cell lines through indirect immunofluorescence. (C) …The results revealed that the mean level of ERβ5 gene expression was lower in advanced clinical stages. ERβ5, estrogen receptor β5. (LI, S et al.)
Dilution used: 1:500