Anti-Follistatin Clone 17/2

Follistatin is a single-chain glycosylated protein that inhibits follicle stimulating hormone (FSH) release. Alternative splicing of Follistatin mRNA yields two isoforms, FS315 and FS288. FS288 is the main cell-surface form and binds to surface heparin sulphate proteoglycans. Clone 17/2 recognizes recombinant human Follistatin 288, allowing for detection of FSH levels using various analysis methods. This antibody also works in a two site ELISA with Clone 29/9.

Catalogue No. Target Research Areas Applications Size Price (£)
17/2-100UG Follistatin
0.1mg 347.00

Clone 17/2 used to detect immunoreactivity with FS288 by IHC-P

Immunolocalization of FS315 and FS288 to PC3 and LNCaP cell lines. Positive immunoreactivity for FS315 localized to the cytoplasm of the epithelial tumor cell lines LNCaP (A) and PC3 (B) using H10 antibody, no immunoreactivity was present if the antibody was preabsorbed with FS315 peptide (inset Ainset B). Mouse IgG antibody controls were negative (inset Cinset D). (McPherson, S.J. et al. 1999)

Concentration used: 10 μg/mL
Dilution used: 1:50

Western Blots showing binding of 17/2 to isoforms of FS288

A. Protein bands corresponding to the approximate 31 and 35K molecular weight isoforms of FS were detected in PC3 media (lane 2) and lanes containing hrFS288 protein (lane 1). (McPherson, S.J. et al. 1999)

Concentration used: 1 μg/mL