Follistatin is a single-chain glycosylated protein that inhibits follicle stimulating hormone (FSH) release. Alternative splicing of Follistatin mRNA yields two isoforms, FS315 and FS288. FS288 is the main cell-surface form and binds to surface heparin sulphate proteoglycans. 29/9 is a clone raised against recombinant Fst 288, and is used in combination, commonly as the capture, with antibody 17/2 in a two site ELISA for the detection of Follistatin.
| Catalogue No. | Target | Research Areas | Applications | Size | Price (£) |
|---|---|---|---|---|---|
| 29/9-100UG | Follistatin (FS288) |
Endocrinology Cancer Fertility Hepatology Apoptosis |
ELISA | 0.1mg | 347.00 |
Form in stock: IgG, purified – 1.0 mg/mL. Also available as unpurified supernatant.
Host: Mouse
Specificity: Raised to human Follistatin (Fst) 288 and recognizes both human Fst 288 and 315.
Human Histology positive control: Testis or Ovaries
Fusion partner: Spleen cells immunised from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Storage: Store at +4°C or -20°C. Avoid repeated freezing and thawing.
Shelf life: 18 months from date of dispatch.
Regulatory/ Restrictions: For research and commercial purposes.
| Application | Suggested Dilution |
|---|---|
| ELISA | In combination with clone 17/2, acting as the capture antibody |
- ⇓ Evans, L. “Development, Validation and Application of an Ultra-sensitive Two-site Enzyme Immunoassay for Human Follistatin.” Journal of Endocrinology 156.2 (1998): 275-82.
- ⇓ Hughes, Robin D., and Lee W. Evans. “Activin A and Follistatin in Acute Liver Failure.” European Journal of Gastroenterology & Hepatology 15.2 (2003): 127-31.
- ⇓ D’antona, D. “Increased Maternal Serum Activin A but Not Follistatin Levels in Pregnant Women with Hypertensive Disorders.” Journal of Endocrinology 165.1 (2000): 157-62.
- ⇓ Anderson, R. A., L. W. Evans, D. S. Irvine, M. A. Mcintyre, N. P. Groome, and S. C. Riley. “Follistatin and Activin A Production by the Male Reproductive Tract.” Human Reproduction 13.12 (1998): 3319-325.
- ⇓ Stephen J. McPherson, Sally L. Mellor, Hong Wang, Lee W. Evans, Nigel P. Groome, Gail P. Risbridger; Expression of Activin A and Follistatin Core Proteins by Human Prostate Tumor Cell Lines. Endocrinology 1999; 140 (11): 5303-5309.
- ⇓ Yuen, M.-F., S. Norris, L. W. Evans, P. G. Langley, and R. D. Hughes. “Transforming Growth Factor-Beta 1, Activin and Follistatin in Patients with Hepatocellular Carcinoma and Patients with Alcoholic Cirrhosis.” Scandinavian Journal of Gastroenterology 37.2 (2002): 233-38.
- ⇓ Menon, U., Riley, S. C., Thomas, J., Bose, C., Dawnay, A., Evans, L. W., Groome, N. P. and Jacobs, I. J. (2000), Serum inhibin, activin and follistatin in postmenopausal women with epithelial ovarian carcinoma. BJOG: An International Journal of Obstetrics & Gynaecology, 107: 1069–1074.
- ⇓ Cuckle, H. S., I. Sehmi, R. Jones, and L. W. Evans. “Maternal Serum Activin A and Follistatin Levels in Pregnancies with Down Syndrome.” Prenatal Diagnosis 19.6 (1999): 513-16.
- ⇓ Fowler, P. A., L. W. Evans, N. P. Groome, A. Templeton, and P. G. Knight. “A Longitudinal Study of Maternal Serum Inhibin-A, Inhibin-B, Activin-A, Activin-AB, Pro-alphaC and Follistatin during Pregnancy.” Human Reproduction 13.12 (1998): 3530-536.
- ⇓ Riley, S. C., C. Balfour, N. C. Wathen, T. Chard, L. W. Evans, N. P. Groome, and E. M. Wallace. “Follistatin and Activin A in Extra-embryonic Coelomic and Amniotic Fluids and Maternal Serum in Early Pregnancy.” Human Reproduction 13.9 (1998): 2624-628.
Antibody 29/9 used to detect Fst levels using two-site ELISA with antibody 17/2
Dose–response curves for various human biological fluid samples containing follistatin using the optimized ELISA procedure. (Evans, L et al.)
Antibody 29/9 used to detect Fst levels using two-site ELISA with antibody 17/2
Serum levels of follistatin in patients with acute liver failure… (Hughes, R,D et al.)
Antibody 29/9 used to detect Fst levels using two-site ELISA with antibody 17/2
Maternal serum levels of (A) Follistatin… (C) the ratio between activin A and follistatin in healthy controls (open symbols) and in patients with gestational diseases during the third trimester… (D’antona, D)
Antibody 29/9 used to detect Fst levels using two-site ELISA with antibody 17/2
Concentration of Follistatin in seminal plasma collected from normal (n=20), post-vasectomy (n=20) and oligo/azoospermic men (n=20). (Anderson, R. A)