Anti-Follistatin Clone H10

Follistatin is a single-chain glycosylated protein that inhibits follicle stimulating hormone (FSH) release.  Alternative splicing of Follistatin mRNA yields two isoforms, FS315 and FS288. FS315 is considered the main circulating form of Follistatin. Clone H10 recognizes an epitope on the C-terminal region of human Follistatin 315, allowing for detection of FSH levels using various analysis methods. ELISA experiments showed that H10 interacts with FS315 and does not interact with FS288. (McPherson, S.J. et al. 1999)

Catalogue No. Target Research Areas Applications Size Price (£)
H10-100UG Follistatin
(raised to FS315)
Endocrinology
Cancer
Fertility
Immunology
Neurosciences
Stem cell
ELISA
WB
IHC
0.1mg 300.00

Clone PPG5/25 used to detect expression of ERβ5 in cancer tissue by IHC-P

Image caption: Immunoexpression of ERs in endometrial cancers. Tissues were classified as well (A-D), moderately (E-H) or poorly (J-M) differentiated; …immunoexpression of ERβ1, 2, 5 was readily detected (K. L, M). Inserts in panels K, L, and M show negative controls for ERβ1, ERβ2 and ERβ5 antibodies respectively generated using primary antibodies pre-absorbed with specific peptides used for immunisation. Asterisks (*) label the stromal compartment that was well defined in the well differentiated cancers. (Collins, F et al.)
Dilution used: 1:75

Clone H10 used to detect immunoreactivity with FS315 by IHC-P.

Immunolocalization of FS315 and FS288 to PC3 and LNCaP cell lines. Positive immunoreactivity for FS315 localized to the cytoplasm of the epithelial tumor cell lines LNCaP (A) and PC3 (B) using H10 antibody, no immunoreactivity was present if the antibody was preabsorbed with FS315 peptide (inset Ainset B). Mouse IgG antibody controls were negative (inset Cinset D). (McPherson, S.J. et al. 1999)

Concentration used: 6.75 μg/mL

Clone H10 used to detect expression of FS315 in TM cells by IHC-P

FST 315 expression in NTM (C) and GTM (G) tissues… (Fitzgerald, A et al.)

Dilution used: 1:100

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