GDF9 is plays a vital role in ovarian folliculogenesis, follicle development and fertility. Clone 72B can be used in assays to detect GDF9 expression and help diagnose ovarian disorders. (Gilchrist, R.B. et al.), (Simpson, C et al.)
| Catalogue No. | Target | Research Areas | Applications | Size | Price (£) |
|---|---|---|---|---|---|
| 72B-100UG | GDF9 | Endocrinology Fertility |
ELISA WB |
0.1mg | 347.00 |
Form in stock: IgG, purified – 1.0 mg/mL. Also available as unpurified supernatant.
Host: Rat
Specificity: N-terminal of GDF9, corresponding to sequence KKPLGPASFNLSEYFC.
Human Histology positive control: Ovary
Fusion partner: Spleen cells from immunised outbred rats were fused with cells of the mouse NS0 myeloma cell line.
Storage: Store at +4°C or -20°C. Avoid repeated freezing and thawing.
Shelf life: 18 months from date of dispatch.
Regulatory/ Restrictions: For research and commercial purposes.
| Application | Suggested Dilution |
|---|---|
| ELISA | 500ng / 50μL2 |
| Western Blot | Assay dependent |
- ⇓ R.B. Gilchrist, L.J. Ritter, M. Cranfield, L.A. Jeffery, F. Amato, S.J. Scott, S. Myllymaa, N. Kaivo-Oja, H. Lankinen, D.G. Mottershead, N.P. Groome, O. Ritvos; Immunoneutralization of Growth Differentiation Factor 9 Reveals It Partially Accounts for Mouse Oocyte Mitogenic Activity. Biol Reprod 2004; 71 (3): 732-739.
- ⇓ Courtney M. Simpson, David M. Robertson, Sara L. Al-Musawi, Derek A. Heath, Kenneth P. McNatty, Lesley J. Ritter, David G. Mottershead, Robert B. Gilchrist, Craig A. Harrison, Peter G. Stanton; Aberrant GDF9 Expression and Activation Are Associated With Common Human Ovarian Disorders. J Clin Endocrinol Metab 2014; 99 (4): E615-E624.
Clone 53/1 used to detect GDF9 expression by ELISA
Image caption: GDF9 ELISA. A GDF9 ELISA was developed to measure the amount of GDF9 in HEK-293T conditioned medium. Recombinant mouse GDF9 (●) was used as a standard, and the specificity of the assay was assessed using a range of TGF-β family members; wild-type human GDF9 (♦), human GDF9 L40V (◊), human BMP15 (□), human activin A (○), and human TGF-β3 (▾). Dilutions of concentrated media from cells transfected with empty vector, pcDNA3.1 (r), were included as controls. The ELISA has a specificity of less than 0.1%, with a sensitivity of 0.2 ng/mL. Values represent mean ± SEM in duplicate, from a representative experiment. (Simpson, C et al.)