Anti-Growth Differentiation Factor 9B Clone 28A

BMP15, also known as GDF9B, plays a crucial role in the regulation of fertility. Clone 28A has been used to detect BMP15 expression and biosynthesis in various carcinoma progression studies.

Catalogue No. Target Research Areas Applications Size Price (£)
28A-100UG GDF9B Endocrinology
Developmental biology
0.1mg 347.00

Form in stock: IgG, purified – 1.0 mg/mL. Also available as unpurified supernatant.
Host: Mouse
Specificity: Recognizes sequence SAEVTASSSKHSGPENNQC on the C-terminus of human GDF9B (BMP15).
Fusion partner: Spleen cells from immunised T/O mice were fused with cells of the SP2/0 myeloma cell line.
Storage: Store at +4°C or -20°C. Avoid repeated freezing and thawing.
Shelf life: 18 months from date of dispatch.
Regulatory/ Restrictions: For research and commercial purposes.

Application Suggested Dilution
ELISA Assay dependent
Western Blot 1:50001
Immunocytochemistry Assay dependent
Immunoblotting 1:100003
  1. Sara L. Al-Musawi, Kelly L. Walton, Derek Heath, Courtney M. Simpson, Craig A. Harrison; Species Differences in the Expression and Activity of Bone Morphogenetic Protein 15. Endocrinology2013; 154 (2): 888-899.
  2. Sudiman, J., Sutton-McDowall, M. L., Ritter, L. J., White, M. A., Mottershead, D. G., Thompson, J. G., & Gilchrist, R. B. (2014). Bone Morphogenetic Protein 15 in the Pro-Mature Complex Form Enhances Bovine Oocyte Developmental Competence. PLoS ONE, 9(7), e103563.
  3. Pulkki, M.M., Myllymaa, S., Pasternack, A., Lun, S., Al-Qahtani, Korchynskyi, O., Groome, N., Juengel, J.L., Laitinen, M., Ritvos, O., Mottershead, D.G. 2011 The bioactivity of human BMP15 is sensitive to C-terminal modification : characterization of the purified untagged processed mature region. Mol. Cell. Endocrinol. 332: 106-15.

Clone 28A used to detect prodomain mutations in human MBP15 biosynthesis by Western Blot

Samples were detected under reduced conditions using a human-specific BMP15 mAb (A) or an anti-FLAG M2 antibody (B). C, Five nonconserved residues (boldface type, underlined) within the α1-helix of the hBMP15 prodomain (Leu44, Glu46, Glu47, Leu49, and Glu50) were substituted with the corresponding mBMP15 residues (Ser43, Leu45, Asp46, Ala48, and Lys49) using site-directed mutagenesis. (Al-Musawi, S et al.)
Dilution used: 1:5000

Clone 28A used to purifiy and quantify human BMP15 by Western Blot

B. The processed mature region of pro-mature BMP15 was quantified by Western blotting [mab28 monoclonal antibody [42]] using the mature region of hBMP15 (R&D Systems) as a standard. Lanes 1–4: mature region of BMP15 (R&D Systems); 200, 100, 50 and 10 ng, respectively. Lanes 5–7: decreasing doses of the purified BMP15 pro-mature complex. (Sudiman, J et al.)

Clone 28A used to detect BMP15 in HEK-293T cell lines by Immunoblotting

Image caption: (B) Recombinant proteins produced from stable HEK-293T cell lines and subjected to SDS–PAGE immunoblotting (ECL). 1: BMP15wt, 2: BMP15 form 1, 3: BMP15 form 2 (with C-terminal Flag tag), 4: GDF9 wt (recognised by the GDF9 specific mAb-53 (Gilchrist et al., 2004b)), 5: TGF-β 200 ng. All samples were reduced with 10 mM DTT before running into the gels. The specific BMP15 mAb-28 reveals the 16–17 kDa mature human BMP15 protein. The mAb-28 does not cross-react with GDF9, showing the specificity of the antibody to BMP15. (Pulkki M,M et al.)
Dilution used: 1:10000