Anti-Growth Differentiation Factor 9B Clone 28A

BMP15, also known as GDF9B, plays a crucial role in the regulation of fertility. Clone 28A has been used to detect BMP15 expression and biosynthesis in various carcinoma progression studies.

Catalogue No. Target Research Areas Applications Size Price (£)
28A-100UG GDF9B Endocrinology
Cancer
Fertility
Developmental biology
ELISA
WB
ICC
IB
0.1mg 300.00

Clone 28A used to detect prodomain mutations in human MBP15 biosynthesis by Western Blot

Samples were detected under reduced conditions using a human-specific BMP15 mAb (A) or an anti-FLAG M2 antibody (B). C, Five nonconserved residues (boldface type, underlined) within the α1-helix of the hBMP15 prodomain (Leu44, Glu46, Glu47, Leu49, and Glu50) were substituted with the corresponding mBMP15 residues (Ser43, Leu45, Asp46, Ala48, and Lys49) using site-directed mutagenesis. (Al-Musawi, S et al.)
Dilution used: 1:5000

Clone 28A used to purifiy and quantify human BMP15 by Western Blot

B. The processed mature region of pro-mature BMP15 was quantified by Western blotting [mab28 monoclonal antibody [42]] using the mature region of hBMP15 (R&D Systems) as a standard. Lanes 1–4: mature region of BMP15 (R&D Systems); 200, 100, 50 and 10 ng, respectively. Lanes 5–7: decreasing doses of the purified BMP15 pro-mature complex. (Sudiman, J et al.)

Clone 28A used to detect BMP15 in HEK-293T cell lines by Immunoblotting

Image caption: (B) Recombinant proteins produced from stable HEK-293T cell lines and subjected to SDS–PAGE immunoblotting (ECL). 1: BMP15wt, 2: BMP15 form 1, 3: BMP15 form 2 (with C-terminal Flag tag), 4: GDF9 wt (recognised by the GDF9 specific mAb-53 (Gilchrist et al., 2004b)), 5: TGF-β 200 ng. All samples were reduced with 10 mM DTT before running into the gels. The specific BMP15 mAb-28 reveals the 16–17 kDa mature human BMP15 protein. The mAb-28 does not cross-react with GDF9, showing the specificity of the antibody to BMP15. (Pulkki M,M et al.)
Dilution used: 1:10000

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