Higher levels of inhibin α have been associated with a higher risk of cancer progression and recurrence. Clone PO 12/8 is useful in detecting inhibin α levels, especially in prostate cancer cells.
| Catalogue No. | Target | Research Areas | Applications | Size | Price (£) |
|---|---|---|---|---|---|
| PO12/8-100UG | Inhibin α | Endocrinology Cancer Fertility Cell biology |
ELISA IHC |
0.1mg | 347.00 |
Form in stock: IgG, purified – 1.0 mg/mL. Also available as unpurified supernatant.
Host: Mouse
Specificity: Synthetic peptide corresponding to epitope region aa73-96 of the αC region of α subunit of inhibin A. (Robertson D.M. et al.)
Human Histology positive control: Prostate tissue
Fusion partner: Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Storage: Store at +4°C or -20°C. Avoid repeated freezing and thawing.
Shelf life: 18 months from date of dispatch.
Regulatory/ Restrictions: For research and commercial purposes.
| Application | Suggested Dilution |
|---|---|
| ELISA | Assay dependent |
| IHC | 90 μg/ml3 |
- ⇓ Balanathan, P., Williams, E. D., Wang, H., Pedersen, J. S., Horvath, L. G., Achen, M. G., … Risbridger, G. P. (2009). Elevated level of inhibin-α subunit is pro-tumourigenic and pro-metastatic and associated with extracapsular spread in advanced prostate cancer. British Journal of Cancer, 100(11), 1784–1793.
- ⇓ D.M. Robertson, T. Stephenson, N. Cahir, A. Tsigos, E. Pruysers, P.G. Stanton, N. Groome, P. Thirunavukarasu, Development of an inhibin α subunit ELISA with broad specificity, Molecular and Cellular Endocrinology, Volume 180, Issues 1–2, 30 July 2001, Pages 79-86, ISSN 0303-7207.
- ⇓ G.P. Risbridger, E.M.A. Ball, H. Wang, S.L. Mellor, D.M. Peehl, Re-evaluation of inhibin α subunit as a tumour suppressor in prostate cancer, Molecular and Cellular Endocrinology, Volume 225, Issues 1–2, 15 October 2004, Pages 73-76, ISSN 0303-7207.
Clone PO 12/8 used to stain prostate cancer cells and detect levels of inhibin a by IHC-P
INHα expression in clinical specimens and its association to prostate disease. Immunohistochemical staining of INHα in primary prostate tumours from PCa patients with (A–D) organ-confined (negative) and (E–H) metastatic disease (positive). (Balanathan, P et al.)
Clone PO 12/8 used to stain prostate cancer cells and detect levels of inhibin a by IHC-P
Immunostaining in biopsy specimens from men with prostate cancer in regions of non-malignant (a, c, e) or malignant (b, d, f) tissue… Panels e and f show the up-regulation of inhibin α subunit immunoreactivity in malignant compared to non-malignant regions of tissue using the monoclonal antibody, PO#12. (Risbridger G.P et al.)
Dilution used: 90 μg/ml