Anti-Myelin Basic Protein Clone MBP12

Myelin Basic Protein (MBP) is involved in the process of myelination of nerves in the nervous system. MBP Clone 12 recognizes an epitope in the 82-87 region of MBP, useful in clinical diagnosis to detect MBP levels or myelination in human MBP.

Catalogue No. Target Research Areas Applications Size Price (£)
MBP12-100UG Myelin Basic Protein
(region 82-87)
Immunology
Neurosciences
Genetics
Pathology
Stem Cell
FC
ELISA
WB
IHC
IF
ICC
0.1mg 300.00

Clone MBP12 used to detect mouse myelin binding via Flow Cytometry

Incubation of mouse myelin with mAb against myelin components. The binding is visualized by incubating the mAb-myelin with fluorescent labelled conjugates. The fluorescence intensity (FL2) of the myelin was measured using FACScan flow Cytometry. The data are presented as the geo-mean of fluorescence of one representative experiment (n=4). (Van der Goesa, et al.)
Dilution used: 75μg/ml

Clone MBP 12 used to detect myelinated structures in MS plaques by IHC-P

Serial sections of paraffin-embedded MS tissue immunostained with anti-EP(A), clone26(B), clone2(C), clone14(D), clone12(E), or clone22(F) .Notice that only abnormal myelin tissues strongly stained by anti-EP, whereas all other antibodies strongly stain the normal myelin surrounding the plaque area. See Materials and Methods for details. (Matsuo, A et al.)
Dilution used: 1:100,000

Clone 12 used to detect MBP in myelinating cell cultures using Immunofluorescence

Immunohistochemistry of transplanted neurospheres demonstrate that cyto-GFP labelled cells form early and mature myelinating oligodendrocytes.
Cyto-GFP-expressing neurospheres were transplanted into a shiverer mouse 3, 7 or 15 days post-transplantation, and 10 µm thick frozen sections were cut and immunolabelled with antibodies to GFP and MBP (Ioannidou, K et al.)
Dilution used: 1:500

Clone 12 used to detect expression of MBP in mouse brain lysates via Western Blot and ICC

A. MBP protein can only be detected by immunocytochemistry in differentiated Schwann cells B, Western Blots of undifferentiated and differentiated primary Schwann cells show MBP protein only present in differentiated Schwann cells
C, MBP and sncRNA715-specific RT-PCR on RNA extracted from undifferentiated or differentiated primary Schwann cells (Müller, C et al.)
Dilution used: 1:50 (ICC) 1:500 (WB)

Clone 12 used to detect MBP in mouse oligodendrocytes using Immunofluorescence and Western Blot

α-Syn impairs oligodendrocyte maturation. Oligodendrocyte progenitor cells were either untreated (Co) or incubated with rh α-Syn (10 μg/ml) 2 h after plating for 3 or 6 days. Cells were subjected to immunocytochemistry using antibodies: a anti-acetylated α-tubulin (green) and anti-MBP (red); b anti-proteoglycan NG-2 (green) and anti-MBP (red). Nuclei were stained with DAPI (blue). Scale bar: 20 μm. c Exogenously applied α-Syn led to an increase in NG-2 and a decrease in MBP levels. (Grigoletto, J et al.)
Dilution used: 1:1500 (WB) 1:200 (ICC)

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