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Anti-Proteolipid Protein Clone PLPC1

Proteolipid protein (PLP) is the major myelin protein of the CNS and plays an important role in the formation and maintenance of myelin. Mutations in PLP gene can lead to dysmyelinating diseases. Clone PLPC1 is used in the detection of PLP by various analysis methods.

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Catalogue No. Target Research Areas Applications Size Price (£)
PLPC1-100UG Proteolipid protein Immunology
Neuroscience
Infectious diseases
Cardiology		 FC
WB
IHC
IF
ICC		 0.1mg 347.00

Form in stock: IgG, purified – 1.0 mg/mL. Also available as unpurified supernatant.
Host: Mouse
Specificity: Synthetic peptide GRGTKF that recognizes the C terminal region of myelin proteolipid protein
Human Histology positive control: Brain
Fusion partner: Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Storage: Store at +4°C or -20°C. Avoid repeated freezing and thawing.
Shelf life: 18 months from date of dispatch.
Regulatory/ Restrictions: For research and commercial purposes.

Application Suggested Dilution
Flow Cytometry 75μg/ml1
WB 1:25024
IHC 1:200-1:30007-22
IF Assay dependent
ICC 1:1000-1:500022,23
  1. ⇓ Van der Goesa, Annette, et al. (2000) The Role of Anti-Myelin (Auto)-Antibodies in the Phagocytosis of Myelin by Macrophages. Journal of Neuroimmunology, 101, 61-67.
  2. ⇓ Yao, B., Hametner, S., van Gelderen, P., Merkle, H., Chen, C., Lassmann, H., … Bagnato, F. (2014). 7 Tesla Magnetic Resonance Imaging to Detect Cortical Pathology in Multiple Sclerosis. PLoS ONE, 9(10), e108863.
  3. ⇓ Betzer, C., Movius, A. J., Shi, M., Gai, W.-P., Zhang, J., & Jensen, P. H. (2015). Identification of Synaptosomal Proteins Binding to Monomeric and Oligomeric α-Synuclein. PLoS ONE, 10(2), e0116473.
  4. ⇓ Höftberger, R., Leisser, M., Bauer, J., & Lassmann, H. (2015). Autoimmune encephalitis in humans: how closely does it reflect multiple sclerosis ? Acta Neuropathologica Communications, 3, 80.
  5. ⇓ Massa, P. T., Ropka, S. L., Saha, S., Fecenko, K. L., & Beuler, K. L. (2002). Critical Role for Protein Tyrosine Phosphatase SHP-1 in Controlling Infection of Central Nervous System Glia and Demyelination by Theiler’s Murine Encephalomyelitis Virus. Journal of Virology, 76(16), 8335–8346.
  6. ⇓ Van Horssen, J., Singh, S., van der Pol, S., Kipp, M., Lim, J. L., Peferoen, L., … Amor, S. (2012). Clusters of activated microglia in normal-appearing white matter show signs of innate immune activation. Journal of Neuroinflammation, 9, 156. IHC, Dilution used 1:500
  7. ⇓ Stephan Bramow, Josa M. Frischer, Hans Lassmann, Nils Koch-Henriksen, Claudia F. Lucchinetti, Per S. Sørensen, Henning Laursen; Demyelination versus remyelination in progressive multiple sclerosis. Brain 2010; 133 (10): 2983-2998. IHC, Dilution used 1:1000
  8. ⇓ Gijs Kooij, Mark R. Mizee, Jack van Horssen, Arie Reijerkerk, Maarten E. Witte, Joost A.R. Drexhage, Susanne M.A. van der Pol, Bert van het Hof, George Scheffer, Rik Scheper, Christine D. Dijkstra, Paul van der Valk, Helga E. de Vries; Adenosine triphosphate-binding cassette transporters mediate chemokine (C-C motif) ligand 2 secretion from reactive astrocytes: relevance to multiple sclerosis pathogenesis. Brain 2011; 134 (2): 555-570. IHC, Dilution used 1:500
  9. ⇓ Coulpier, F., L. Decker, B. Funalot, J. M. Vallat, F. Garcia-Bragado, P. Charnay, and P. Topilko. “CNS/PNS Boundary Transgression by Central Glia in the Absence of Schwann Cells or Krox20/Egr2 Function.” Journal of Neuroscience 30.17 (2010): 5958-967. IHC, Dilution used 1:400
  10. ⇓ Shanu F. Roemer, Joseph E. Parisi, Vanda A. Lennon, Eduardo E. Benarroch, Hans Lassmann, Wolfgang Bruck, Raul N. Mandler, Brian G. Weinshenker, Sean J. Pittock, Dean M. Wingerchuk, Claudia F. Lucchinetti; Pattern-specific loss of aquaporin-4 immunoreactivity distinguishes neuromyelitis optica from multiple sclerosis. Brain 2007; 130 (5): 1194-1205. IHC, Dilution used 1:1000
  11. ⇓ Jeroen J. G. Geurts, PhD, Lars Bö, MD, PhD, Stefan D. Roosendaal, MD, Thierry Hazes, MSc, Richard Daniëls, MSc, Frederik Barkhof, MD, PhD, Menno P. Witter, PhD, Inge Huitinga, PhD, Paul van der Valk, MD, PhD; Extensive Hippocampal Demyelination in Multiple Sclerosis. J Neuropathol Exp Neurol 2007; 66 (9): 819-827. IHC, Dilution used 1:3000
  12. ⇓ Kooi, E.-J., Prins, M., Bajic, N., Beliën, J. A. M., Gerritsen, W. H., van Horssen, J., … Geurts, J. J. G. (2011). Cholinergic imbalance in the multiple sclerosis hippocampus. Acta Neuropathologica, 122(3), 313–322. IHC, Dilution used 1:500
  13. ⇓ Seewann, A., E.- J. Kooi, S. D. Roosendaal, P. J. W. Pouwels, M. P. Wattjes, P. Van Der Valk, F. Barkhof, C. H. Polman, and J. J. G. Geurts. “Postmortem Verification of MS Cortical Lesion Detection with 3D DIR.” Neurology 78.5 (2012): 302-08. IHC, Dilution used 1:3000
  14. ⇓ Kooi, E.-J., E. M. M. Strijbis, P. Van Der Valk, and J. J. G. Geurts. “Heterogeneity of Cortical Lesions in Multiple Sclerosis: Clinical and Pathologic Implications.” Neurology 79.13 (2012): 1369-376. IHC, Dilution used 1:500
  15. ⇓ Thomas Skripuletz, Diane Hackstette, Katharina Bauer, Viktoria Gudi, Refik Pul, Elke Voss, Katharina Berger, Markus Kipp, Wolfgang Baumgärtner, Martin Stangel; Astrocytes regulate myelin clearance through recruitment of microglia during cuprizone-induced demyelination. Brain 2013; 136 (1): 147-167. IHC, Dilution used 1:500
  16. ⇓ Bagnato, F., Hametner, S., Yao, B., van Gelderen, P., Merkle, H., Cantor, F. K., … Duyn, J. H. (2011). Tracking iron in multiple sclerosis: a combined imaging and histopathological study at 7 Tesla. Brain, 134(12), 3599–3612. IHC, Dilution used 1:1000
  17. ⇓ Gilmore, C. P., Bö, L., Owens, T., Lowe, J., Esiri, M. M. and Evangelou, N. (2006), Spinal Cord Gray Matter Demyelination in Multiple Sclerosis—A Novel Pattern of Residual Plaque Morphology. Brain Pathology, 16: 202–208. IHC, Dilution used 1:3000
  18. ⇓ Jatana, Manu, Inderjit Singh, Avtar K. Singh, and Dorothea Jenkins. “Combination of Systemic Hypothermia and N-acetylcysteine Attenuates Hypoxic-Ischemic Brain Injury in Neonatal Rats.” Pediatric Research 59.5 (2006): 684-89. IHC, Dilution used 1:200
  19. ⇓ Giuseppina Maccarrone, Sandra Nischwitz, Sören-Oliver Deininger, Joachim Hornung, Fatima Barbara König, Christine Stadelmann, Christoph W. Turck, Frank Weber, MALDI imaging mass spectrometry analysis—A new approach for protein mapping in multiple sclerosis brain lesions, Journal of Chromatography B, Volume 1047, 15 March 2017, Pages 131-140, ISSN 1570-0232. IHC, Dilution used 1:500
  20. ⇓ Chuang, T.-Y., Guo, Y., Seki, S. M., Rosen, A. M., Johanson, D. M., Mandell, J. W., … Gaultier, A. (2016). LRP1 expression in microglia is protective during CNS autoimmunity. Acta Neuropathologica Communications, 4, 68. IHC, Dilution used 1:500
  21. ⇓ Manuela Cerina, Venu Narayanan, Kerstin Göbel, Stefan Bittner, Tobias Ruck, Patrick Meuth, Alexander M. Herrmann, Martin Stangel, Viktoria Gudi, Thomas Skripuletz, Thiemo Daldrup, Heinz Wiendl, Thomas Seidenbecher, Petra Ehling, Christoph Kleinschnitz, Hans-Christian Pape, Thomas Budde, Sven G. Meuth, The quality of cortical network function recovery depends on localization and degree of axonal demyelination, Brain, Behavior, and Immunity, Volume 59, January 2017, Pages 103-117, ISSN 0889-1591. IHC, Dilution used 1:500
  22. ⇓ Janssen, Katharina, Mira Rickert, Tim Clarner, Cordian Beyer, and Markus Kipp. “Absence of CCL2 and CCL3 Ameliorates Central Nervous System Grey Matter But Not White Matter Demyelination in the Presence of an Intact Blood Brain Barrier.” Molecular Neurobiology 53.3 (2015): 1551-564. IHC and ICC, Dilution used 1:1500
  23. ⇓ Haider, L., Fischer, M. T., Frischer, J. M., Bauer, J., Höftberger, R., Botond, G., … Lassmann, H. (2011). Oxidative damage in multiple sclerosis lesions. Brain, 134(7), 1914–1924. ICC, Dilution used 1:1000
  24. ⇓ Cheng, Clara M, et al. “Biochemical and Morphometric Analyses Show That Myelination in the Insulin-like Growth Factor 1 Null Brain Is Proportionate to Its Neuronal Composition.” The Journal Of Neuroscience 18.15 (1998): 5673-681. WB, Dilution used 1:250
  25. ⇓ Jaśkiewicz, Ewa, et al. (2005) Expression of Recombinant Forms of Human 21.5 KDa Myelin Basic Protein and Proteolipid Protein in CHO Cells. Acta Biochimica Polonica, 52, (4/2005) 863-866. FC
  26. ⇓ Garcia-Vallejo,J.J., Ilarregui,J.M., Kalay,H., Chamorro,S., Koning,N., Unger,W.W.,van Kooyk, Y. (2014). CNS myelin induces regulatory functions of DC-SIGN-expressing, antigen-presenting cells via cognate interaction with MOG. Journal of Experimental Medicine, 211(7), 1457-1483. FC

Clone PLPC1 used to detect mouse myelin binding via Flow Cytometry

Incubation of mouse myelin with mAb against myelin components. The binding is visualized by incubating the mAb-myelin with fluorescent labelled conjugates. The fluorescence intensity (FL2) of the myelin was measured using FACScan flow Cytometry. The data are presented as the geo-mean of fluorescence of one representative experiment (n=4). (Van der Goesa, et al.)
Dilution used: 75μg/ml

Clone PLPC1 used for the detection of PLP in brain tissue by Western Blot

Human (G-I) brain extracts were analyzed by immunoblotting using antibodies against antigens selected among the monomer and oligomer binding proteins… (Betzer, C et al.)
Dilution used: 1:250

Clone PLPC1 used for the detection of PLP in brain tissue by IHC-P

A) Type-I NL with demyelination of the whole width of the cortex (#) and adjacent WM (*). (B) Type-II intracortical lesion evolving around a vessel. (C) Type-III subpial NL. Demyelination spreads from the pial surface until cortical layer 3. (Yao, B et al.)
Dilution used: 1:1000

Clone PLPC1 used for the detection of PLP in brain tissue by ICC

l: The active lesion edge of the cortical lesions contains numerous macrophages with PLP reactive myelin degradation products… (Höftberger, R et al.)
Dilution used: 1:1000

Clone PLPC1 used for the detection of PLP in brain tissue by IF

Image caption: … PLP (B, D, and F; TRITC) in the ventral cervical spinal cords of me/me (A to D) mice 5 days after inoculation with TMEV… (Massa, P. T et al.)