CYP450 Aromatase Clone H4

CYP450 Aromatase is part of the CYP19A1 family involved in the aromatization of androgens to estrogens, a highly conserved mechanism amongst mammals. Clone H4 recognizes a conserved epitope on the CYP450 aromatase, allowing for detection of aromatase levels using various analysis methods. (Turner et al. 2002)

Catalogue No. Target Research Areas Applications Size Price (£)
H4-100UG Aromatase Endocrinology
Cancer
Fertility
Veterinary science
WB
IHC
IF
ICC
0.1mg 300.00

Clone H4 used to detect aromatase in human tissues by IHC-P.

A: Intense aromatase immunostaining in IGCN cells.
B: Placental-like alkaline phosphatase staining of IGCN basal cells
C: Strong aromatase immunoreactivity of interstitial Leydig cells in normal testis (Lc)
D: Intense immunostaining of luteal cells (Luc) in ovarian tissue.(Rago, V et al. 2005)

Dilution used: 1:50

Binding of H4 to a 55 kDa protein on the Cytochrome P450 aromatase protein by Western Blot.

On Western blots, a single protein of _55 kDa was recognised by the monoclonal antibody raised against a peptide within the P450 aromatase protein (A). No signal was seen on blots incubated with antibody that had been pre-absorbed with immunising peptide (B). Extracts were prepared from the following tissues: lane 1, human placenta; lane 2, marmoset placenta; lane 3, marmoset ovary; lane 4, ovary from pregnant rat; lanes 5 and 6, ovaries from two rats killed at pro-oestrus.(Turner et al. 2002)

Dilution used: 1:5000

Clone H4 used to detect aromatase in pig sperm by Immunofluorescence.

B) P450arom red brilliant light in the proximal tail of sperm with a diffuse labelling in the distal tail…. (Rago, V et al. 2007)

Dilution used: 1:100

Clone H4 used to detect aromatase in stromal cells by ICC.

Immunocytochemical localization of aromatase in the stromal cells of an endometriotic lesion (B) and in granulosa cells of a developing follicle (C, positive control). The histology of this lesion (A). st = stromal cells. (Fazleabas, AT et al.)

Dilution used: 1:75

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