2019 nCoV/COVID19 Nucleocapsid protein (N) Recombinant, His-tag (100 µg)

Accession: NCOVNP
Source: DNA sequence encoding the COVID-19 Nucleocapsid protein domain, amino acids[1-419] (accession# YP_009724397.2) including a C-terminal His tag, was expressed in CHO Cells.
Molecular weight: The recombinant 2019-nCoV Nucleocapsid protein is consisting of 431 amino acid residues. Due to glycosylation migrates as an approximately 60 kDa protein on SDS-PAGE.
Purity: >95%, as determined by SDS-PAGE and HPLC.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation: Recombinant nCoV-2019 Nucleocapsid protein was lyophilized from 0.2 µm filtered PBS, pH 7.4.
Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Shipping: Lyophilized Protein- shipped at ambient temperature.
Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20°C.Repeated freeze-thaw cycles should be avoided.
Usage: This product is for Research Use Only. It may not be used for therapeutics or diagnostic purposes.

nCoV/COVID19 Nucleocapsid Recombinant Protein, His-tag Datasheet

Coronaviruses have a positive-sense RNA genome with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express Nucleocapsid (N) protein.

The N protein is a structural protein that binds to the coronavirus RNA genome, thus creating a shell (or capsid) around the enclosed nucleic acid. Besides
1. interacts with the viral membrane protein during viral assembly
2. assists in RNA synthesis and folding
3. plays a role in virus budding
4. affects host cell responses, including cell cycle and translation.

Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. N protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. It is a highly immunogenic phosphoprotein, also implicated in viral genome replication, and in modulating cell signalling pathways. It is chosen as a diagnostic tool, due to the conservation of N protein sequence and its strong immunogenicity.