2019 nCoV/COVID19 Spike protein S1 Recombinant-His tag (100 µg)

£364.00

100 µg

SKU: BSV-COV-PR-36

DNA sequence encoding extracellular fragment [16-675] of nCoV Spike S1
including a C-terminal polyHis was expressed in HEK cells.

Predicted Molecular Mass: 90 kDa
2019-nCov amino acids: 16-675 aa (Accession No. QIC53204.1)

Source: DNA sequence encoding extracellular fragment[16-675] of NCOV Spike S1 [accession# QIC53204.1] including a C terminal polyHis was expressed in HEK cells.
Molecular weight: Recombinant NCOV S1 encodes the NCOV spike glycoprotein S1 sequence. This molecule has a calculated mass of approximately 76kDa. Recombinant S1 migrates due to glycosylation as an approximately 120 kDa protein under reducing conditions in SDS-PAGE.
Species: 2019-nCoV, SARS-CoV-2
Activity: The activity was tested by binding Human ACE2-Fc in functional ELISA assay, the calculated EC50 was determined to be 0.1μg/ml.
Purity: >95%, as determined by SDS-PAGE and HPLC.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation: Recombinant COVID-19 Spike S1 was lyophilized from 0.2 µm filtered PBS solution pH7.4.
Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Tag: C-terminal His-Tag
Host: HEK293 cells
Formulation: Lyophilised from 0.2μg filtered PBS solution pH 7.4.
Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage: This product is for Research Use Only. It may not be used for therapeutics or diagnostic purposes.

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. It has been reported that 2019-nCoV can infect the human Respiratory Epithelial cells through interaction with the human ACE2 receptor. The S protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. So, S protein has a key role in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Known receptors binding S1 are ACE2, Angiotensin-Converting Enzyme 2; DPP4, Dipeptidyl Peptidase-4; APN, Aminopeptidase N; CEACAM, Carcinoembryonic antigen-related cell adhesion molecule 1; Sia, Sialic acid; O-ac Sia, O-acetylated Sialic acid.

The S protein is essential for both host specificity and viral infectivity. The term ‘peplomer’ is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. Besides, the S protein is known to be essential in the binding of the virus to the host cell at the advent of the infection process.

The main functions for the S protein are summarized as:
Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

2019-nCoV S1-His tagged (coating at 0.5μg well) binding with Human ACE2 Fc. The linear range was found to be 0.1-5μg/ml.