2019 nCoV/COVID19 Spike protein S1 Recombinant-His tag (500 µg)

Source: DNA sequence encoding extracellular fragment[16-675] of NCOV Spike S1 [accession# QIC53204.1] including a C terminal polyHis was expressed in HEK cells.
Molecular weight: Recombinant NCOV S1 encodes the NCOV spike glycoprotein S1 sequence. This molecule has a calculated mass of approximately 76kDa. Recombinant S1 migrates due to glycosylation as an approximately 120 kDa protein under reducing conditions in SDS-PAGE.
Species: 2019-nCoV,SARS-CoV-2
Activity: The activity was tested by binding Human ACE2-Fc in functional ELISA assay, the calculated EC50 was determined to be 0.1μg/ml.
Tag: His-tag.
Host: HEK293 cells
Purity: >95%, as determined by SDS-PAGE and HPLC.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation: Recombinant COVID-19 Spike S1 was lyophilized from 0.2 µm filtered PBS solution pH7.4.
Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20°C.
Usage: This product is for Research Use Only. It may not be used for therapeutics or diagnostic purposes.

2019 nCoV/COVID19 Recombinant SARS-CoV-2 Spike Glycoprotein (S1), His-Tag Datasheet

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. It has been reported that 2019-nCoV can infect the human Respiratory Epithelial cells through interaction with the human ACE2 receptor. The S protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. So, S protein has a key role in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Known receptors binding S1 are ACE2, Angiotensin-Converting Enzyme 2; DPP4, Dipeptidyl Peptidase-4; APN, Aminopeptidase N; CEACAM, Carcinoembryonic antigen-related cell adhesion molecule 1; Sia, Sialic acid; O-ac Sia, O-acetylated Sialic acid.

The S protein is essential for both host specificity and viral infectivity. The term ‘peplomer’ is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. Besides, the S protein is known to be essential in the binding of the virus to the host cell at the advent of the infection process.

The main functions for the S protein are summarized as:
Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.