Anti-ACE2 (Human) Monoclonal Antibody (Blocking) [Clone AC384] (Preservative Free) (100 μg)

£276.00

100 μg

SKU: BSV-COV-AB-26

Anti-ACE2 (Human) Monoclonal Antibody (Blocking) [Clone AC384]

Anti-ACE2 (Human) monoclonal antibody purified by Protein G.

Concentration: 1 mg/ml

Clonality: Monoclonal

Clone: AC384

Specificity: Recognizes human ACE2.
Formulation: Liquid. In PBS.
Isotype: Mouse IgG1κ
Immunogen: The immunogen used to generate this antibody corresponds to Recombinant Human ACE2 protein.
Clonality: Monoclonal
Clone name: AC384
Buffer: PBS
Purity: Affinity purified by Protein G
Label/Conjugates: Preservative Free
Alternative names: SARS-CoV Spike, 2019-nCoV Spike, SARS-CoV-2 Spike, COVID-19 Spike.
Shipping: Shipped on Blue Ice.
Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at +4°C. For long-term storage, aliquot and store at -20°C or below (it can remain stable for at least 1 year after receipt). After opening prepare aliquots and store at -20°C. Avoid multiple freeze-thaw cycles.
Regulatory/Restrictions: For Research Use Only.

Application: Suggested Dilution
Western Blot: 1:2000-1:10000
ELISA: 1:2000-1:10000
Functional Application: Blocks the binding of human ACE2 to the Spike protein of SARS-CoV-2.

Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Clone AC384 inhibits the binding of ACE2 (human) to the Spike protein of SARS-CoV-2 by ELISA.
Binding of ACE2 (human) to the Spike protein of SARS-CoV-2 is inhibited by the anti-ACE2 monoclonal antibody (BSV-COV-AB-26). Spike is coated on an ELISA plate at 1ug/ml. ACE2 (human) mAb (blocking) (BSV-COV-AB-26) or an unrelated mAb control are added together with 500ng/ul of ACE2 (human). After incubation for 1h at RT, the binding was detected using an anti-FLAG antibody (HRP).
Dilution started at: 40μg/ml