Anti-ACE2 (Human) Monoclonal Antibody [Clone AC18F] (Biotin) (50μg)

£244.00

50 μg

SKU: BSV-COV-AB-21

Anti-ACE2 (Human) Monoclonal Antibody [Clone AC18F] (Biotin)

Anti-ACE2 (Human) monoclonal antibody purified by Protein G.

Concentration: 1 mg/ml

Clonality: Monoclonal

Clone: AC18F

Specificity: Recognizes human ACE2. Does not detect recombinant human ACE2 that has a tag at the C-terminus of the protein, due to the recognized C-terminal epitope.
Formulation: Liquid. 0.2μm-filtered solution in PBS, pH 7.4. (contains no preservatives)
Isotype: Mouse IgG1κ
Immunogen: The immunogen used to generate this antibody corresponds to ACE2 protein.
Clonality: Monoclonal
Clone name: AC18F
Buffer: PBS
Purity: Affinity purified by Protein G
Label/Conjugates: Biotin
Alternative names: SARS-CoV Spike, 2019-nCoV Spike, SARS-CoV-2 Spike, COVID-19 Spike.
Shipping: Shipped on Blue Ice.
Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at +4°C. For long-term storage, aliquot and store at -20°C or below (it can remain stable for at least 1 year after receipt). After opening prepare aliquots and store at -20°C. Avoid multiple freeze-thaw cycles.
Regulatory/Restrictions: For Research Use Only.

Application: Suggested Dilution
Western Blot: 1:2000-1:10000
Flow Cytometry: 1:1000
ELISA: 1:2000-1:10000

Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Clone AC18F (Biotin) used to detect endogenous human ACE2 by anti-ACE2 (h) by Flow Cytometry.
The line is treated with CellTase solution (Abeomics) before analysis and then stained with antiACE2 (h), mAb Clone AC18F (BSV-COV-AB-21) (red line) or an isotype control, mouse IgG1 (green line) at 1µg/10E6 cells each, revealed with a PE-conjugated goat anti-mouse IgG.

Clone AC18F analysis using anti-ACE2 (human), mAb (AC18F) (Biotin) by Western Blot.
Western Blot of ACE2 monoclonal antibody (BSV-COV-AB-21) at 1:2000 dilution. Lane1: hACE2 (Fc protein), Lane 2: hACE2 (Ecto domain)
Lane 3: Other hGITR (Fc protein), acts as control.
Dilution used: 1:2000