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Recombinant Anti-SARS-CoV-2 Spike Protein S1, Monoclonal Antibody (blocking) [Covi-2] (100 μg)

£328.00

100 μg

SKU: BSV-COV-AB-30

Recombinant Anti-SARS-CoV-2 Spike Protein S1, Monoclonal Antibody (blocking) [Covi-2]

Recombinant Anti-SARS-CoV-2 Spike (S1) Monoclonal Antibody [Covi-2] ≥98% purity by SDS-PAGE.

Concentration: 1 mg/ml

Clonality: Recombinant Monoclonal

Clone: Covi-2

Specificity: Recognizes and binds to recombinant SARS-CoV-2 Spike Protein S1. It blocks the interaction of SARS-CoV-2 Spike Protein S1 (RBD) to human ACE2. Detects a different epitope as anti-SARS-CoV-2 Spike Protein S1, mAb (rec.) (blocking) (Covi-1, BSV-COV-AB-29)
Form in stock: Liquid, IgG, purified – 1mg/mL.
Host: HEK 293 cells
Immunogen: The immunogen used to generate this antibody corresponds to recombinant SARS-CoV-2 Spike Protein S1 receptor binding domain (RBD).
Clonality: Recombinant Monoclonal
Clone name: Covi-2
Isotype:Human IgG1
Formulation: Liquid. In PBS.
Buffer: PBS
Purity: ≥98% (SDS-PAGE)
Label/Conjugates: Preservative Free
Alternative names: 2019-nCoV Spike Protein S1
Shipping: Shipped on Blue Ice.
Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage, store at +4°C. For long-term storage, store at -20°C. After opening, prepare aliquots and store at -20°C. Avoid freeze/ thaw cycles.
Regulatory/Restrictions: For Research Use Only.

Application: Suggested Dilution
Western Blot: 1:1000
ELISA: Assay Dependent
Functional Application: Blocking

Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

ELISA Clone Covi-2 inhibits the binding of hACE2 to the Spike protein of SARS-CoV-2 by ELISA.
SARS-CoV-2 Spike Protein S1 is coated on an ELISA plate at 1ug/ml. SARS-CoV-2 Spike Protein S1, mAb (blocking) [Covi-2] (BSV-COV-AB-30) or an unrelated recombinant mAb (control) is added together with 500ng/ul of hACE (Biotin). After incubation for 1h at 37oC, the binding is detected using Streptavidin (HRP).
Dilution starting at: 40ug/ml with a two-fold serial dilution
ELISA Clone Covi-2 assaying with SARS-CoV-2 S1 Protein by ELISA.
ELISA binding assay with SARS-CoV-2 S1 Protein (RBD) (His) using anti-SARS-CoV-2 Spike Protein (S1), mAb [Covi-2] (BSV-COV-AB-30) (blue line) and an unrelated antibody as negative control (red line).