SARS-CoV-2 S1 Protein ELISA is a research use only sandwich ELISA designed for the quantitative measurement of SARS-CoV-2 S1 Protein in saliva. It uses Avacta’s proprietary Affimer™ technology. The assay employs dual capture Affimer™ reagents (BSV-COV-AVA60001 & BSV-COV-AVA60003) binding to the SARS-CoV-2 S1 Receptor Binding Domain with a non-competing biotinylated SARS-CoV-2 S1 detector Affimer™ reagent.
Capture Affimer Reagent 2 can be used to detect and quantify SARS-CoV-2 S1 Protein in saliva with the sandwich ELISA method. It is mixed with Capture Affimer Reagent 1 (BSV-COV-60001) at the beginning of the protocol to coat the 96-well plate.
96-well plates are coated with dual capture anti SARS-CoV-2 S1 Affimer™ reagents that capture SARS-CoV-2 S1 protein in the sample. After washing away unbound materials, captured SARS-CoV-2 S1 protein is detected by a non-competing biotinylated SARS-CoV-2 S1 detector Affimer™ reagent and streptavidin conjugated to horse radish peroxidase (HRP). After washing, the chromogenic substrate 3,3’,5,5’-tetramethylbenzidine (TMB) is added. Colour reaction is stopped by 0.5M H2SO4. The amount of SARS-CoV-2 S1 captured inside the wells is proportional to the colour intensity generated in the coupled oxidation-reduction reaction.
Step 1. Affimer™ reagent coating – Prepare a capture reagent mix of 1 µg/mL Capture Affimer (BSV-COV-AVA60001) + 1 µg/mL Capture Affimer (BSV-COV-AVA60003).
Step 2. Detection – Biotinylated Affimer™ reagent – Prepare 1 µg/mL biotinylated detection Affimer reagent (BSV-COV-AVA60005) in 1x casein buffer.
Learn more in the IFU