Description
The CleanPlex® Single-Indexed PCR Primers for Ion Torrent™ are high-quality ready-to-use PCR primers for Ion Torrent library construction. They are compatible and designed for use with all CleanPlex NGS Panels to constructed targeted libraries for sequencing on an Ion Torrent NGS platform.
Primer Sequences
Each sample is indexed by two pairs of Indexed PCR Primers for sequencing on Ion Torrent platforms. XXXXXXXXX denotes the index region of the primer.
Indexed PCR Primers
5’-CCATCTCATCCCTGCGTGTCTCCGACTCAGXXXXXXXXXCGATTTCAGACGTGTGCTCTTCCGATC*T-3′
5’-CCATCTCATCCCTGCGTGTCTCCGACTCAGXXXXXXXXXCGATCCTACACGACGCTCTTCCGATC*T-3′
Universal PCR Primers
5’-CCGCTTTCCTCTCTATGGGCAGTCGGTGATTTCAGACGTGTGCTCTTCCGATC*T-3′
5’-CCGCTTTCCTCTCTATGGGCAGTCGGTGATCCTACACGACGCTCTTCCGATC*T-3′
Note:
Please refer to CleanPlex NGS Panel User Guide for instructions on using CleanPlex Single-Indexed PCR Primers for Ion Torrent
Primer Sequences:
Each sample is indexed by a pair of Indexed PCR Primers for sequencing on MGISEQ platforms.
XXXXXXXXXX denotes the index region of the primer.
Universal Primer
5′-Phos-GAACGACATGGCTACGATCCGACT*T-3’
Indexed Primers
5′-TGTGAGCCAAGGAGTTGXXXXXXXXXXTTGTCTTCCTAAGACCGCTTGGCCTCCGACT*T-3’
Note:
Please refer to the CleanPlex for MGI NGS Panel User Guide for instructions on using CleanPlex for MGI Single-Indexed PCR Primers