The CleanPlex UMI Lung Cancer Panel is a targeted resequencing assay designed for rapid and high confident detection of low-frequency variants across the hotspot regions of 23 genes associated with lung cancer. Based on CleanPlex UMI technology, this panel is specifically designed to work with cell-free DNA (cfDNA) and FFPE DNA for precise analysis of tumor DNA in liquid biopsy and tumor profiling applications. The panel is powered by Paragon Genomics’ CleanPlex UMI Technology which incorporates novel unique molecular identifiers (UMIs) to enable correction of PCR and sequencing errors. Starting with just 20 ng of DNA, sequencing-ready libraries can be prepared using a single-tube workflow in just 3.5 hours. Low-frequency variants below 0.5% allele frequency can be confidently detected with high specificity using just 30 ng of DNA.
- Relevant Gene Content
Interrogate 23 genes containing variants that are frequently mutated in lung cancer using a hotspot panel designed to work with cell-free DNA (cfDNA)
- Fast, Single-Tube Workflow
Generate high-quality molecular-barcoded and target-enriched NGS libraries in just 3.5 hours using a three-step, single-tube protocol
- Enhanced Error Correction
Remove PCR and sequencing errors with powerful error correction enabled by novel unique molecular identifiers (UMIs) that can distinguish the two strands of DNA.
- High-Confidence Variant Detection
Detect low-frequency variants below 0.5% allele frequency with high specificity using just 30 ng of DNA