50 μg
2019 Coronavirus SARS-CoV-2 Nucleocapsid Protein
The recombinant SARS-CoV-2 (2019-nCoV) nucleocapsid (N) Protein was expressed and purified from in vitro cell culture of HEK293 cells, with a C-terminal DYKDDDDK tag.
Predicted Molecular Mass: SARS-CoV-2 (2019-nCoV) nucleocapsid (N) predicts a molecular mass of 49.7 kDa.
2019-nCoV Amino Acids: The amino acid sequence of recombinant protein was derived from a full-length sequence of accession# YP_009724397.1 / QHD43423.1.
Applications: Antigens, Western, ELISA and other in vitro binding or in vivo functional assays, and protein-protein interaction studies.
Purity: >90 % by SDS-PAGE.
Tag: DYKDDDDK tag.
Endotoxin: Endotoxin level is < 0.1 ng/μg of protein (<1 EU/μg)
Expressed Host: HEK293 cells.
Species: 2019-nCoV
Predicted Molecular Mass: SARS-CoV-2 (2019-nCoV) nucleocapsid (N) predicts a molecular mass of 49.7 kDa.
2019-nCoV Amino Acids: The amino acid sequence of recombinant protein was derived from a full-length sequence of accession# YP_009724397.1 / QHD43423.1.
Formulation: Protein formulated in a solution of 0.1M glycine, 50 mM Tris.Cl, pH7.4, 150mM NaCl.
Shipping: Liquid Protein- shipped on Dry Ice.
Storage: Upon receipt, unopened vial can be stored at -80°C for over 12 months. Repeated freeze-thaw cycles should be avoided. Besides, the product can be aliquoted in the smaller size of working aliquots with the desired buffer and concentration, stored at or below -20°C and remain stable for 3 to 4 weeks.
Regulatory/Restrictions: For Research Use Only.
Coronaviruses have a positive-sense RNA genome with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express Nucleocapsid (N) protein.
The N protein is a structural protein that binds to the coronavirus RNA genome, thus creating a shell (or capsid) around the enclosed nucleic acid. Besides
1. interacts with the viral membrane protein during viral assembly
2. assists in RNA synthesis and folding
3. plays a role in virus budding
4. affects host cell responses, including cell cycle and translation.
Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. N protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. It is a highly immunogenic phosphoprotein, also implicated in viral genome replication, and in modulating cell signalling pathways. It is chosen as a diagnostic tool, due to the conservation of N protein sequence and its strong immunogenicity.