50 μg
Recombinant SARS-CoV-2 Spike RBD Protein, human IgG1 Fc tag from Wuhan pneumonia virus (MN908947.3). Expressed and purified from human cells, with a C-terminal Fc tag.
Predicted Molecular Mass: SARS-CoV-2 Spike RBD Protein, human IgG1 Fc tag predicts a molecular mass of approximately 52 kDa.
2019-nCov amino acids: The amino acid sequences of recombinant protein was derived from accession# YP_009724390.1 fused to Glu99-Lys330 region of human IgG1 constant region.
Applications: Antigens, Western, ELISA and other in vitro binding or in vivo functional assays, and protein-protein interaction studies.
Purity: >90 % by SDS-PAGE.
Tag: Fc-tag
Predicted Molecular Mass: SARS-CoV-2 Spike RBD Protein, human IgG1 Fc tag predicts a molecular mass of approximately 52 kDa.
2019-nCov Amino Acids: The amino acid sequences of recombinant protein was derived from accession# YP_009724390.1 fused to Glu99-Lys330 region of human IgG1 constant region.
Endotoxin: Endotoxin level is < 0.1 ng/μg of protein (<1 EU/μg)
Expressed Host: Expressed and purified from in vitro cell culture of HEK293 cells
Species: 2019-nCoV
Formulation: Protein formulated in a sterile solution of PBS buffer, pH 7.2 without any preservatives.
Storage: The product is shipped with dry ice. Upon receipt, unopened vial can be stored at -80°C for over 12 months. Avoid repeated freeze/thaw cycles. Also the product can be aliquoted in the smaller size of working aliquots with the desired buffer and concentration, and stored at or below -20°C stable for 3 to 4 weeks.
Regulatory/ Restrictions: For Research Use Only.
The coronavirus Spike protein (S) is a large oligomeric transmembrane protein that mediates coronavirus entry into host cells. It contains S1 and S2 two subunits. Spike S1 mainly contains a receptor binding domain (RBD) that recognizes a variety of host cell surface receptors. S2 contains basic elements responsible for the membrane fusion. The coronavirus first binds to a receptor on the host cell surface through Spike S1 subunit, and then fuses viral and host membranes through Spike S2 subunit.