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  5. LyteStar 2019-nCoV PCR detection kit S (E gene)

LyteStar 2019-nCoV PCR detection kit S (E gene)

£1,150.00

96 reactions

SKU: BSV-qPCR-10

Screening assay for the detection of E-gene.
The LyteStar SARS-CoV-2 RT-PCR Kit S is an in vitro diagnostic test system, based on real-time PCR technology, for the qualitative detection of Sarbecovirus specific RNA (which includes SARS-CoV, SARS-CoV-2 and bat-SARS-related CoVs).
The LyteStar SARS-CoV-2 RT-PCR Kit S consists of an assay that targets the E gene of the SARS-CoV-2 genome (Figure 1). The assay includes a heterologous amplification system (Internal Control) to identify possible RT-PCR inhibition and to confirm the integrity of the reagents of the kit.

Product Name: LyteStar 2019-nCoV PCR Kit S (E gene)
Intended Use: specific detection of Sarbecovirus specific RNA (includes SARS-CoV, SARS-CoV-2 and bat-SARSrelated CoVs) in human respiratory specimens.
Type of assay: screening assay targeting the Sarbecovirus E gene.
Specimen types: Human respiratory specimens

  • Bronchoalveolar lavage
  • Tracheal aspirate
  • Sputumnasopharyngeal
  • Oropharyngeal swabs placed in VTM
  • nasopharyngeal wash/aspirate
  • Nasal wash/aspirate

    • Shipment and Storage:
    • Store all reagents at -20°C upon arrival.
    • Shelf life is 6 months from the manufacturing date.
    • Repeated thawing and freezing should be avoided, as this might affect the performance of the assay. The reagents should be frozen in aliquots, if they are to be used intermittently.
    • Avoid vigorous vortex mixing of Master A, which contains RT enzyme.
    • Protect Master B from light.
    • All frozen reagents should be completely thawed to room temperature beforeuse. Immediately return unused portions to the freezer for storage.
    For research use only!

    Materials Supplied:

  • User Manual 1
  • Master A 192 µl
  • Master B – target E gene 4 x 432 µl
  • Internal Control (IC) 1000 µl
  • Positive Control (PC) – target E gene 250 µl
  • PCR grade water 500 µl

    • Extracted RNA is the starting material for the LyteStar 2019-nCoV RT-PCR Kit S. The quality of the extracted RNA has a profound impact on the performance of the whole test system. It has to be ensured that the nucleic acid extraction system used is compatible with real-time PCR technology.

    Recommended sample preparation:
    The SpinStar Viral Nucleic Acid Kit is designed for the simultaneous purification of viral DNA and RNA from fresh or frozen human serum and plasma, respiratory specimens and stool. The extracted DNA and/or RNA is ready to use for any downstream application where highly purified nucleic acids are required.

    The following nucleic acid extraction kits / systems are suitable for use with the LyteStarTM 2019-nCoV RT-PCR Kit S:
    • SpinStarTM Viral Nucleic Acid Kit (ADT Biotech)
    • QIAamp® MinElute Virus Spin Kit (Qiagen)
    • QIAamp® Viral RNA Mini Kit (Qiagen)
    • HighPure® Viral Nucleic Acid Kit (Roche)
    • QIAsymphony® (Qiagen)
    • NucliSENS® easyMag (bioMérieux)
    • MagNA Pure 96 System (Roche)
    Alternative nucleic acid extraction systems and kits might also be appropriate. The suitability of the nucleic acid extraction procedure for use with LyteStarTM 2019-nCoV RT-PCR Kit S has to be validated by the user.
    If using a spin column based sample preparation procedure including washing buffers containing ethanol, an additional centrifugation step for 10 min at approximately 17000 x g (~ 13000 rpm), using a new collection tube, prior to the elution of the nucleic acid is highly recommended.