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Forward primer for SARS-CoV-2 detection (50 qPCR reactions)

20µL

SKU: BCC-19nCoVR_F50

BaseClick provides primers and probes recommended by the Centers for Disease Control and Prevention to researchers for the in vitro detection of the novel coronavirus (2019-nCoV). The primers and probes are designed for the universal detection of SARS-like coronaviruses (N3 assay) and for the specific detection of novel 2019-nCoV (N1 and N2 assays).
RP – all clinical samples should be tested for human RNAse P (RNP) gene to assess specimen quality.
If you are interested in primers and probes to create a Real-Time RT-PCR panel in your lab, follow the panel instructions from the CDC to get more instructions for use.

Catalog No.DescriptionSequence (5’-3’)LengthLabel
BCC-19nCoVR_F50Forward Primer for 50 qPCR reactionsAGA TTT GGA CCT GCG AGC G19-
BCC-19nCoVR_R50Reverse Primer for 50 qPCR reactionsGAG CGG CTG TCT CCA CAA GT20-
BCC-19nCoVR_P50Probe for 50 qPCR reactionsFAM– TTC TGA CCT GAA GGC TCT GCG CG –BHQ123FAM / BHQ1
BCC-19nCoVR_S50Set of R Primers & Probe for 50 qPCR reactionsAGA TTT GGA CCT GCG AGC G
GAG CGG CTG TCT CCA CAA GT
FAM– TTC TGA CCT GAA GGC TCT GCG CG –BHQ1
19
20
23
-
-
FAM / BHQ1
BCC-19nCoVR_F100Forward Primer for 100 qPCR reactionsAGA TTT GGA CCT GCG AGC G19-
BCC-19nCoVR_R100Reverse Primer for 100 qPCR reactionsGAG CGG CTG TCT CCA CAA GT20-
BCC-19nCoVR_P100Probe for 100 qPCR reactionsFAM– TTC TGA CCT GAA GGC TCT GCG CG –BHQ123FAM / BHQ1
BCC-19nCoVR_S100Set of R Primers & Probe for 100 qPCR reactionsAGA TTT GGA CCT GCG AGC G
GAG CGG CTG TCT CCA CAA GT
FAM– TTC TGA CCT GAA GGC TCT GCG CG –BHQ1
19
20
23
-
-
FAM / BHQ1

These sequences are intended to be used for the purposes of respiratory virus surveillance and research. The recipient agrees to use them in compliance with all applicable laws and regulations.

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