20µL
BaseClick provides primers and probes recommended by the Centers for Disease Control and Prevention to researchers for the in vitro detection of the novel coronavirus (2019-nCoV). The primers and probes are designed for the universal detection of SARS-like coronaviruses (N3 assay) and for the specific detection of novel 2019-nCoV (N1 and N2 assays).
RP – all clinical samples should be tested for human RNAse P (RNP) gene to assess specimen quality.
If you are interested in primers and probes to create a Real-Time RT-PCR panel in your lab, follow the panel instructions from the CDC to get more instructions for use.
| Catalog No. | Description | Sequence (5’-3’) | Length | Label |
|---|---|---|---|---|
| BCC-19nCoVR_F50 | Forward Primer for 50 qPCR reactions | AGA TTT GGA CCT GCG AGC G | 19 | - |
| BCC-19nCoVR_R50 | Reverse Primer for 50 qPCR reactions | GAG CGG CTG TCT CCA CAA GT | 20 | - |
| BCC-19nCoVR_P50 | Probe for 50 qPCR reactions | FAM– TTC TGA CCT GAA GGC TCT GCG CG –BHQ1 | 23 | FAM / BHQ1 |
| BCC-19nCoVR_S50 | Set of R Primers & Probe for 50 qPCR reactions | AGA TTT GGA CCT GCG AGC G GAG CGG CTG TCT CCA CAA GT FAM– TTC TGA CCT GAA GGC TCT GCG CG –BHQ1 | 19 20 23 | - - FAM / BHQ1 |
| BCC-19nCoVR_F100 | Forward Primer for 100 qPCR reactions | AGA TTT GGA CCT GCG AGC G | 19 | - |
| BCC-19nCoVR_R100 | Reverse Primer for 100 qPCR reactions | GAG CGG CTG TCT CCA CAA GT | 20 | - |
| BCC-19nCoVR_P100 | Probe for 100 qPCR reactions | FAM– TTC TGA CCT GAA GGC TCT GCG CG –BHQ1 | 23 | FAM / BHQ1 |
| BCC-19nCoVR_S100 | Set of R Primers & Probe for 100 qPCR reactions | AGA TTT GGA CCT GCG AGC G GAG CGG CTG TCT CCA CAA GT FAM– TTC TGA CCT GAA GGC TCT GCG CG –BHQ1 | 19 20 23 | - - FAM / BHQ1 |
These sequences are intended to be used for the purposes of respiratory virus surveillance and research. The recipient agrees to use them in compliance with all applicable laws and regulations.