Recombinant Human ACE2 Protein (H374N, and H378N), human IgG1 Fc tag (50 μg)

Applications: Antigens, Western Blot, ELISA, other in vitro binding or in vivo functional assays, and Protein-Protein Interaction studies.
Purity: >90% as determined by SDS-PAGE and Coomassie Blue staining.
Endotoxin: Endotoxin level is < 0.1 ng/μg of protein (<1 EU/μg)
Expressed Host: Expressed and purified from in vitro cell culture of HEK293 cells.
Species: 2019-nCoV, SARS-CoV-2
Tag C-terminal Fc-tag
Molecular Mass: Predicted MW of this product is ~ 97.8 kDa. It runs higher on SDS-PAGE under the reduced condition due to post-translational modification when expressed and secreted from mammalian cells.
nCoV Amino Acids: The amino acid sequences of recombinant protein was derived from accession# YP_009724390.1 fused to Glu99-Lys330 region of human IgG1 constant region.
Formulation: Protein formulated as 1 mg/mL in a solution of PBS buffer, pH 7.2, without any preservatives.
Shipping: Liquid Protein- shipped on Dry Ice.
Storage: Upon receipt, unopened vial can be stored at -80°C for over 12 months. Repeated freeze-thaw cycles should be avoided. Besides, the product can be aliquoted in the smaller size of working aliquots with the desired buffer and concentration, stored at or below -20°C and remain stable for 3 to 4 weeks.
Regulatory/Restrictions: For Research Use Only.

Recombinant Human Angiotensin-Converting Enzyme (ACE2) Protein (H374N, and H378N), human IgG1 Fc tag Datasheet

Recombinant Human ACE2 Protein (H374N, and H378N), human IgG1 Fc tag

The Angiotensin-Converting Enzyme 2 (ACE2) belongs to the Angiotensin-Converting Enzyme (ACE) family of Dipeptidyl Carboxydipeptidases. ACE2 cleaves the Angiotensin I and Angiotensin II to counterbalancing ACE activity in the regulation of renin angiotensin system (RAS). ACE2 also plays a pivotal role in the regulation of cardiovascular, renal and reproductive systems. Besides, it is a functional receptor for the Spike glycoprotein (S) of SARS-CoV, HCoV-NL63, and SARS-CoV-2, which causes the pandemic of the respiratory diseases (COVID-19).

ACE2 extracellular domain (18-615) binds to the SARS Coronavirus 2 (Cov-2) receptor binding domain (RBD). It is expressed in CHO cells and purified to greater than 95% purity. As a result it is a very useful tool in search of COVID19 conditions.