Recombinant Human ACE2 Protein, human IgG1 Fc tag (50 μg)

£1,014.00

50 μg

SKU: LTP-V003

Recombinant Human Angiotensin-Converting Enzyme 2 (ACE2)

Recombinant protein of the extracellular domain (Gln18-Ser740) of Homo Sapiens Angiotensin Converting Enzyme 2 (ACE2), transcript variant 2 (NM_021804), with a human IgG1 Fc tag.

Predicted Molecular Mass: Recombinant Human ACE2 Protein, human IgG1 Fc tag predicts a molecular mass of ~110 kDa.
2019-nCoV Amino Acids: The amino acid sequence of the recombinant protein was derived from Glu99-Lys330 region of human IgG1 constant region (Accession Number. YP_009724390.1 ).

Applications: Antigens, Western Blot, ELISA, other in vitro binding or in vivo functional assays, and Protein-Protein interaction studies.
Purity: >90 % by SDS-PAGE.
Tag C-terminal His-Tag.
Endotoxin: Endotoxin level is < 0.1 ng/μg of protein (<1 EU/μg)
Expressed Host: Expressed and purified from in vitro cell culture of HEK293 cells.
Species: 2019-nCoV, SARS-CoV-2
Predicted Molecular Mass: Recombinant Human ACE2 Protein, human IgG1 Fc tag predicts a molecular mass of 110 kDa.
2019-nCoV Amino Acids: The amino acid sequence of Recombinant Protein was derived from Glu99-Lys330 region of human IgG1 constant region.
Formulation: Protein formulated as 0.4 mg/mL in a solution of PBS buffer, pH7.2, without any preservatives.
Shipping: Liquid protein- shipped on Dry Ice.
Storage: Upon receipt, unopened vial can be stored at -80°C for over 12 months. Repeated freeze-thaw cycles should be avoided. Besides, the product can be aliquoted in the smaller size of working aliquots with the desired buffer and concentration, stored at or below -20°C and remain stable for 3 to 4 weeks.
Regulatory/Restrictions: For Rearch Use Only.

The Angiotensin-Converting Enzyme 2 (ACE2) belongs to the Angiotensin-Converting Enzyme (ACE) family of Dipeptidyl Carboxydipeptidases. ACE2 cleaves the Angiotensin I and Angiotensin II to counterbalancing ACE activity in the regulation of renin angiotensin system (RAS). ACE2 also plays a pivotal role in the regulation of cardiovascular, renal and reproductive systems. Besides, it is a functional receptor for the Spike glycoprotein (S) of SARS-CoV, HCoV-NL63, and SARS-CoV-2, which causes the pandemic of the respiratory diseases (COVID-19).

ACE2 extracellular domain (18-615) binds to the SARS Coronavirus 2 (Cov-2) receptor binding domain (RBD). It is expressed in CHO cells and purified to greater than 95% purity. As a result it is a very useful tool in search of COVID19 conditions.