50 μg
Recombinant protein of the receptor binding domain (RBD) of SARS-CoV-2 (2019-nCoV) Spike S1 from Wuhan pneumonia virus (MN908947.3), with a polyhistidine tag at its C-terminus.
Predicted Molecular Mass: Recombinant SARS-CoV-2 Spike S1 RBD Protein predicts a molecular mass of ~29.5 kDa.
2019-nCov amino acids: The amino acid sequences of recombinant protein was derived from accession# YP_009724390.1.
Applications: Antigens, Western, ELISA and other in vitro binding or in vivo functional assays, and protein-protein interaction studies
Purity: >95 % by SDS-PAGE.
Tag: C-terminal His-Tag
Endotoxin: Endotoxin level is < 0.1 ng/μg of protein (<1 EU/μg)
Expressed Host: Expressed and purified from in vitro cell culture of HEK293 cells
Species: 2019-nCoV
Predicted Molecular Mass: Recombinant SARS-CoV-2 Spike S1 RBD Protein predicts a molecular mass of ~29.5 kDa.
2019-nCov amino acids: The amino acid sequences of recombinant protein was derived from accession# YP_009724390.1.
Formulation: Purified protein formulated in a sterile solution of PBS buffer, pH7.2.
Storage: The product is shipped with dry ice. Upon receipt, unopened vial can be stored at -80°C for over 12 months. Avoid repeated freeze/thaw cycles. Also the product can be aliquoted in the smaller size of working aliquots with the desired buffer and concentration, and stored at or below -20°C stable for 3 to 4 weeks.
Regulatory/Restrictions: For research use only.
The coronavirus Spike protein (S) is a large oligomeric transmembrane protein that mediates coronavirus entry into host cells. It contains S1 and S2 two subunits. Spike S1 mainly contains a receptor binding domain (RBD) that recognizes a variety of host cell surface receptors. S2 contains basic elements responsible for the membrane fusion. The coronavirus first binds to a receptor on the host cell surface through Spike S1 subunit, and then fuses viral and host membranes through Spike S2 subunit.