SARS Coronavirus Nucleoprotein, His-Tag (HEK293)

£2,060.00

500 μg

SKU: BSV-COV-PR-29

SARS nCoV/COVID19 Recombinant Nucleoprotein (N)

SARS Coronavirus Nucleoprotein is a recombinant antigen manufactured in HEK293 cells and it is used in vaccine research and immunoassay development. The protein was purified by immobilised metal affinity and ion exchange chromatography.

Predicted Molecular Mass: The SARS nCoV/COVID19  Recombinant Nucleoprotein  predicts a molecular mass of 48 kDa.

2019-nCoV Amino Acids: 2-422 aa (Accession No. AAR87518.1)

Tag: 6x His-tag
Expressed Host: Expressed and purified from in vitro cell culture of HEK293 cells.
Species: 2019-nCoV
2019-nCov amino acids: The amino acid sequences of the Recombinant Protein was derived from accession N AAR87518.1, AA2-422.
Presentation and buffer: 50mM HEPES pH8.0, 280mM NaCl.
Concentration: 1.30 μg/μl
Purification: Immobilised method affinity and ion exchange chromatography.
Shipping: Liquid protein- shipped on Dry Ice.
Storage: Unopened vials can be stored at -80°C. Repeated freeze-thaw cycles should be avoided.
Regulatory/Restrictions: For Research Use Only.

Coronaviruses have a positive-sense RNA genome with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express Nucleocapsid (N) protein.

The N protein is a structural protein that binds to the coronavirus RNA genome, thus creating a shell (or capsid) around the enclosed nucleic acid. Besides
1. interacts with the viral membrane protein during viral assembly
2. assists in RNA synthesis and folding
3. plays a role in virus budding
4. affects host cell responses, including cell cycle and translation.

Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. N protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. It is a highly immunogenic phosphoprotein, also implicated in viral genome replication, and in modulating cell signalling pathways. It is chosen as a diagnostic tool, due to the conservation of N protein sequence and its strong immunogenicity.