SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (His tag) (100 μg)

Purity:>90 % as determined by SDS-PAGE.
Tag: His-tag
Endotoxin: <1.0 EU per μg protein as determined by the LAL method.
Activity: Testing in progress.
Protein Construction: A DNA sequence encoding the NCP-CoV(2019-nCoV) Nucleocapsid Protein was expressed with a polyhistidine (His) tag at the C-terminus.
Expressed Host: Baculovirus-Insect Cells.
Species: 2019-nCoV
Predicted N Terminal: Met
Predicted Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (His tag) consists of 430 amino acids and predicts a molecular mass of 47.08 kDa
2019-nCoV Amino Acids: Met1 – Ala419 (Gene Accession Number: YP_009724397.2)
Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH8.0, 10% glycerol. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage: Should be stored at -20°C to -80°C immediately upon arrival, under sterile conditions. Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Repeated freeze-thaw cycles should be avoided.
Requlatory/Restrictions: For Research Use Only.

SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (His tag) Datasheet

Coronaviruses have a positive-sense RNA genome with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express Nucleocapsid (N) protein.

The N protein is a structural protein that binds to the coronavirus RNA genome, thus creating a shell (or capsid) around the enclosed nucleic acid. Besides
1. interacts with the viral membrane protein during viral assembly
2. assists in RNA synthesis and folding
3. plays a role in virus budding
4. affects host cell responses, including cell cycle and translation.

Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. N protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. It is a highly immunogenic phosphoprotein, also implicated in viral genome replication, and in modulating cell signalling pathways. It is chosen as a diagnostic tool, due to the conservation of N protein sequence and its strong immunogenicity.