Purity: >95 % as determined by SDS-PAGE.
Endotoxin: <1.0 EU per μg protein as determined by the LAL method.
Activity: The 2019-nCoV Spike Protein (RBD, Fc Tag) can bind with Human ACE2 in functional ELISA assay.
Protein Construction: A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (RBD) was expressed with the Fc region of human IgG1 at the C-terminus.
Expressed Host: HEK293 Cells
Predicted N Terminal: Arg
Predicted Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, Fc Tag) consists of 461 amino acids and predicts a molecular mass of 51.8 kDa.
2019-nCov amino acids: Arg319 – Phe541 (Gene Accession Number: YP_009724390.1)
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Regulatory/Restrictions: For research use only.